Double-stranded siRNA corresponding to the homologous sequence of the Nrf2 or HO-1 gene was used to reduce Nrf2 or HO-1 expression. Lipofectamine RNAiMAX reagent was used to conduct transfections according to the manufacturer’s recommendations. Transfected scrambled siRNA was used as the negative ...
The day of the experiment, 120,000 cells were plated in 12-well dishes and transfected with 30 nM Sox4 siRNA (assay s74178, Thermo Fisher Scientific) or negative control siRNA (AM4620, Thermo Fisher Scientific) in Lipofectamine RNAiMAX (13778-075, Thermo Fisher Scientific) according to the...
a process called A-to-I editing, which is mediated by the adenosine deaminase acting on RNA (ADAR) family of enzymes48,49. Since ADAR editing occurs in double-stranded RNA substrates50,51, we asked whether A-to-I editing sites are enriched among CCRs (Fig...
As exosomes naturally carry RNA between cells, these particles might be useful in gene cancer therapy to deliver therapeutic short interfering RNA (siRNA) to the target cells. Despite the promise of RNA interference (RNAi) for use in therapy, several technical obstacles must be overcome. Exogenous...
For this end, electrophoretic mobility shift assays using a DIG-labeled double-stranded 34-bp oligonucleotide containing the E-box-like SRE-38 of hNIS 5′-flanking region (hNIS E-box-like SRE-38) and in vitro translated nSREBP-1c or nSREBP-2 were performed. Reliability of electrophoretic ...
ARGONAUTE4 control of locus-specific siRNA accumulation and DNA and histone methylation Science, 299 (2003), pp. 716-719 Google Scholar Cited by (0) 3 Lead Contact Recommended articles cannot be displayed at this time. Citations Captures ...
The following double-stranded siRNAs specific to human EloB were purchased from Shanghai GenePharma Co., Ltd (Shanghai, China): EloB-siRNA1 (siEB1), GGGAAGCAGUGCCAAUGAATT; EloB-siRNA2 (siEB2), UGACCAACUCUUGGAUGAUTT; EloB-siRNA3 (siEB3), GACGAUGGCCAAGAGCAGATT. The AllStars Negative...
Notably, transfection of human PBMC with siRNA specific for NLRP3 and PYCARD greatly reduced IL-1β production, confirming that the production of this cytokine is dependent on inflammasome assembly [33]. That IL-1β plays a role in the pathogenesis of AD has been repeatedly shown. To ...
VEGF-A165 was obtained from Sigma-Aldrich (St. Louis, MO; V5765). SiRNA for FUNDC1, IP3R1, VEGFR2, serum response factor (SRF), and controls were obtained from Santa Cruz Biotechnology (sc-36563, sc-91118, sc-42475, and sc-29318; Dallas, Texas). Antibodies against the following protei...
Branched ubiquitin (Ub) chains constitute a sizable fraction of Ub polymers in human cells. Despite their abundance, our understanding of branched Ub function in cell signaling has been stunted by the absence of accessible methods and tools. Here we identify cellular branched-chain-specific binding ...