In simple words, a chemical solution or a chemical mixture is known as a buffer if it contains a weak acid and the conjugate base of the...Become a member and unlock all Study Answers Start today. Try it now Create an account Ask a question Our experts can answer your tough homew...
Pellets were resuspended in 1 mL of Z Buffer (60 mM Na2HPO4, 40 mM NaH2PO4, 10 mM KCl, 1 mM MgSO4, 50 mM β-mercaptoethanol, pH 7.0) and OD600 was measured. Cells were permeabilized with 0.5% toluene and 4.5% ethanol for 5 min at 30 °C in a water bath. To...
1. How does the titration of a diprotic acid differ from a monoprotic acid (like HF)? 2. How does the titration of a weak base with a strong acid differ from weak acid-strong base titration. (Review How does H2O affect NaH2PO4 during an acid titration?
Hearts were immediately immersed and flushed with ice cold buffer (130 mM NaCl, 5.4 mM KCl, 1.4 mM MgCl2, 0.4 mM NaH2PO4, 4.2 mM HEPES) before snap freezing in liquid nitrogen. Hearts were thawed at 4 °C and homogenized to a fine paste using a Moulinette yoghurt maker....
2. 0.02M PBS(pH7.2-pH7.6): Na2HPO4·12H2O 7g, NaH2PO4·2H2O 0.5g, NaCl 9g in 1L of distilled water.3. 0.01M Citrate Buffer: 3g sodium citrate dihydrate (C6H5Na3O7·2H2O) and 0.4g citric acid monohydrate (C6H8O7·H2O) in 1L of distilled water.4. DAB Chromogen Kit....
Frozen cell paste was crushed and resuspended via stirring at 4°C in 500 mL of Lysis Buffer (50mM NaH2PO4 pH 7.8, 400mM NaCl) supplemented with protease inhibitors (cOmplete, EDTA-free, Roche Diagnostics Corporation) and 1250U of benzonase (Invitrogen). The resuspended cell paste was lysed ...
A parental HeLa cell was used as a negative control. Forty-eight hours after transfection, cells were lysed in 1 ml of lysis buffer (6 M guanidinium HCl, 100 mM Na2HPO4, 100 mM NaH2PO4 and 10 mM Tris–HCl (pH 7.8)). After sonication, 90% of the lysates were incubated with 50 ml ...
The composition of the solution was as follows: 1 mmol L−1 NH4NO3, 0.32 mmol L−1 NaH2PO4, 0.51 mmol L−1 K2SO4, 1 mmol L−1 CaCl2, 1.65 mmol L−1 MgSO4, 8.9 µmol L−1 MnSO4, 0.5 µmol L−1 Na2MoO4, 18.4 µmol L−1 H3BO3, 0.14 µmol L−1 ZnSO4,...
At different experimental time point, rats were anesthetized and transcardially perfused with 200 ml normal saline solution rapidly, and fixed in situ by perfusion for 20 min at 4 °C with Zamboni’s solution containing 4% paraformaldehyde, 15% saturated picric acid and 24 mM NaH2PO4/...
The pH of a 0.12 M solution of phosphoric acid (H3PO4) is measured to be 1.59. Use this information to determine the value of Ka for phosphoric acid. A buffer consists of 0.22 M H3PO4 and 0.31 M NaH2PO4. Given that the K values for H3PO4 are, Ka...