34 The lipoproteins isolated in the 1.050 to 1.100-g/mL interval were dialyzed against 20 mmol/L Na2HPO4 , pH 7.3, and applied to a lysine-Sepharose column. After washing the column we eluted Lp(a) with the lysine analog, ε-aminocaproic acid 200 mmol/L in a buffer containing 100 mmol...
All buffer solutions contained 1 mm CaCl2, 1 mmMgCl2, 5 mm NaCl; the pH values were as follows: pH 1.25 and 2 (0.2 m KCl, 0.2 m HCl); pH 2.5 and 3–6 (50 mm citric acid, 100 mmNa2HPO4); pH 7, 7.5, and 8–10 (50 mm Tris/HCl). Purified Ym1 crystals (18 mg/ml in wa...
Pellets were resuspended in 1 mL of Z Buffer (60 mM Na2HPO4, 40 mM NaH2PO4, 10 mM KCl, 1 mM MgSO4, 50 mM β-mercaptoethanol, pH 7.0) and OD600 was measured. Cells were permeabilized with 0.5% toluene and 4.5% ethanol for 5 min at 30 °C in a water bath. To...
In simple words, a chemical solution or a chemical mixture is known as a buffer if it contains a weak acid and the conjugate base of the...Become a member and unlock all Study Answers Start today. Try it now Create an account Ask a question Our experts can answer your tough homew...
Determine the pH of a solution that is 0.10 M in NaH2PO4 and 0.15 M in Na2HPO4. (The Ka for H2PO4− is 6.2×10−8.) Buffer Solution: A weak acid exists in a dissociation equilibrium with hydrogen ion and weak conjugate base products. T...
point, rats were anesthetized and transcardially perfused with 200 ml normal saline solution rapidly, and fixed in situ by perfusion for 20 min at 4 °C with Zamboni’s solution containing 4% paraformaldehyde, 15% saturated picric acid and 24 mM NaH2PO4/ 126 mM Na2HPO4 (pH 7.2)...
experimental time point, rats were anesthetized and transcardially perfused with 200 ml normal saline solution rapidly, and fixed in situ by perfusion for 20 min at 4 °C with Zamboni's solution containing 4% paraformaldehyde, 15% saturated picric acid and 24 mM NaH2PO4/ 126 mM Na2HPO4 (pH ...
2. 0.02M PBS(pH7.2-pH7.6): Na2HPO4·12H2O 7g, NaH2PO4·2H2O 0.5g, NaCl 9g in 1L of distilled water.3. 0.01M Citrate Buffer: 3g sodium citrate dihydrate (C6H5Na3O7·2H2O) and 0.4g citric acid monohydrate (C6H8O7·H2O) in 1L of distilled water.4. DAB Chromogen Kit....
A parental HeLa cell was used as a negative control. Forty-eight hours after transfection, cells were lysed in 1 ml of lysis buffer (6 M guanidinium HCl, 100 mM Na2HPO4, 100 mM NaH2PO4 and 10 mM Tris–HCl (pH 7.8)). After sonication, 90% of the lysates were incubated with 50 ml ...
Each Northern blot was washed 15 min at 65 °C in the hybridization solution (0.25 mNa2HPO4, pH 7.1, 1 mm EDTA, 7% (w/v) SDS) and probed overnight in the hybridization solution at 65 °C with [α-32P]dCTP-labeled probe. The blot was then washed by adding a 65 °C hot wash ...