2. 0.02M PBS(pH7.2-pH7.6): Na2HPO4·12H2O 7g, NaH2PO4·2H2O 0.5g, NaCl 9g in 1L of distilled water.3. 0.01M Citrate Buffer: 3g sodium citrate dihydrate (C6H5Na3O7·2H2O) and 0.4g citric acid monohydrate (C6H8O7·H2O) in 1L of distilled water.4. DAB Chromogen Kit....
1. How does the titration of a diprotic acid differ from a monoprotic acid (like HF)? 2. How does the titration of a weak base with a strong acid differ from weak acid-strong base titration. (Review How does H2O affect NaH2PO4 during an acid titration?
, the brains were excised, and immersed in ice-cold, solution (in mM; 248 sucrose, 2.9 KCl, 2 MgSO4, 1.25 NaH2PO4, 26 NaHCO3, 0.1 CaCl2, 10 glucose, 2.5 kynurenic acid, and 1.0 ascorbic acid; pH 7.4–7.45, with 335–345 mOsm). Coronal sections (300 μm) containing the mPFC ...
1.7 mM NaH2PO4, and 10 mM Na2HPO4, pH 7.2), and ultrapure HNO3was added to lyse the cells. Samples were transferred to glass bottles and then boiled for 30 min at
The Henderson-Hasselbalch equation is used to evaluate pH by using the concentration of acid and salt present in a buffer solution. Answer and Explanation: Given: Volume of the buffer solution is 1.00 L. The concentration of NaHC2O4 is ...
on one side and attached to a small hook in a chamber filled with oxygenated (95% O2/5% CO2) physiological solution comprised of 131 mM NaCl, 5 mM KCl, 1.8 mM CaCl2× 2 H2O, 0.5 mM MgCl2× 6 H2O, 0.4 mM NaH2PO4, 24 mM NaHCO3, and 5.5 mM glucose that was maintained at 37°C...
For RNA analysis, dry seeds were treated by 2.5% sodium hydrochloride for 30 min to facilitate the germination, abundantly rinsed and imbibed for 48 h at 4 C in imbibition buffer (KNO3 5 mM, NaH2PO4 10 mM, K2HPO4 10 mM). Imbibed seeds were plated on a wet filter paper and put in...
Purified His-tagged LigI (1 μg) was incubated with either GST (2 μg) or GST-DCAF7 (2 μg) in 300 μl of buffer containing 50 mm NaH2PO4 (pH 7.4), 250 mm NaCl, and 10 mm imidazole for 1 h on ice, followed by the addition of 15 μl of Ni-nitrilotriacetic acid-agarose bead...
column (Macherey–Nagel, Düren, Germany) perfused with a mobile phase composed of 75 mM NaH2PO4, 4 mM KCl, 20 μM ethylenediamine tetraacetic acid, 1.5 mM sodium dodecyl sulfate, 100 μl l − 1 diethylamine, 12% methanol and 12% acetonitrile adjusted to pH 6.0 using phosphoric acid. Th...
Which one of the following is a buffer solution? At what ratio of weak acid to its conjugate base does the pH equal to the pK_a of the acid-base pair? Explain this in terms of the Henderson-Hasselbalch equation. A buffer solution is made by mixing Na2HPO4 with NaH2PO4....