The GI glucosensing mechanism is unknown. We postulated that glucokinase (GK), a high鈥揗ichaelis constant (K[sub m]) hexokinase expressed in brain areas containing populations of GE and GI neurons, is the controlling step in glucosensing. Double-label in situ hybridization demonstrated neuron-...
In the adult hepatocyte, the high-Km glucose transporter, GLUT 2, and the high-Km hexokinase, glucokinase, are sufficient to ensure glucose supply to the hepa- tocyte for glycogenesis by the direct route (11, 12). In contrast, the fetal rat liver stores glycogen at a rapid rate from d...
Glucokinase (hexokinase D) is a monomeric cytoplasmic enzyme found in the liver and pancreas that serves to regulate glucose levels in these organs. ...Glucokinase is a hexokinase isoenzyme. Can two isoenzymes have the same molecular weight? Yes, this isvery possible- isozymes of the same prote...
Characterization and crystal structure of the thermophilic ROK hexokinase from Thermus thermophilus(ENZYMOLOGY, PROTEIN ENGINEERING, AND ENZYME TECHNOLOGY) We characterized and determined the crystal structure of a putative glucokinase/hexokinase from Thermus thermophilus that belongs to the ROK (bacterial rep...
(G6P), which is further used to produce two ATP molecules. G6P enters into the pentose phosphate pathway to produce NADPH and anabolic intermediates [2]. There are four major hexokinase isoforms in mammalian tissues, including HK1, HK2, HK3, and HK4 (also known as glucokinase), and the ...
coli homolog, is monomeric under the conditions of activity measurements. In fact, a number of monomeric enzymes exhibiting allosteric behavior have been reported of which the most thoroughly characterized is human glucokinase (57, 58). However, glucokinase (also called hexokinase IV), unlike l-...
[32]; the vertebrate hexokinase D is often referred to as glucokinase. Like most aerobic eukaryotes and the amitochondriate parasiteEntamoeba histolytica,Trimastixappears to use hexokinase for the first enzymatic step in glycolysis whereas the amitochondriate 'excavates'Giardia intestinalis,Spironucleus ...
These cells express a glucokinase isoform that limits the entry of glucose into metabolism until its concentration exceeds 5 mM (Matschinsky, 1996). The entry of glucose into muscle and adipose cells is insulin dependent, limiting the availability of glucose to these cells during starvation when ...
aGlcN in its endogenously phosphorylated form, GlcN-6-phosphate, is a well-established inhibitor of both hexokinase4 (EC enzyme number 2.7.1.1) and, in particular, its predominant liver-specific functional isoform, glucokinase5 (EC 2.7.1.2), thereby reflecting the initial step within the enzymati...
(Continued) Aliases First positive article Mendelian disorder CD32; Fc IgG low affinity IIa receptor 50 No Glucagon receptor 51 No Glucokinase; MODY2; hexokinase 4 52 125853, MODY2; 602485, autosomal dominant hyperinsulinism; 601410, permanent neonatal diabetes mellitus G-protein beta, polypeptide ...