According to kinetic and immunological analyses, the free extractable cytosolic fraction contained high K m, hexokinase (glucokinase) and less than 10% of low K m hexokinases, while the hexokinase activity bound to the cell structures represented exclusively low K m isozymes. The total activity of...
Glucose-stimulated insulin secretion is believed to require metabolism of the sugar via a high Kmpathway in which glucokinase (hexokinase IV) is rate-limiting. In this study, we have used recombinant adenoviruses to overexpress the liver and islet isoforms of glucokinase as well as low Km...