The cDNA of Renilla reniformis luciferase (ruc) has been cloned and used successfully as a marker gene in a variety of transgenic species. Similarly, the transfer and expression of green fluorescent protein (GFP) cDNA (gfp) and its mutants from Aequorea victoria resulted in high levels of GFP...
The ATR-CHK1 DNA damage response pathway becomes activated by the exposure of RPA-coated single-stranded DNA (ssDNA) that forms as an intermediate during DNA damage and repair, and as a part of the replication stress response. Here, we identify ZNF827 as a component of the ATR-CHK1 kinase...
Fig. 6: ZFAND3 regulates gene expression and is part of a nucleus-specific protein interactome involved in RNA metabolism, processing, and transcription. a RNAseq analysis of HI GSCs (n = 3 per condition) identified 58 common DEGs from two KD clones (shZFAND3-1 and shZFAND3-2) vs...
The GerW Protein Is Not Involved in the Germination of Spores of Bacillus Species subtilis gerW gene is expressed only during sporulation in developing spores, and that GerW is essential for L-alanine germination of B. subtilis spores ... J Cruz-Mora,A Pérez-Valdespino,S Gupta,... - 《...
A genome-wide genetic screen in human KBM7 cells was performed to uncover host factors used by PE, several of which were confirmed by CRISPR/Cas9-gene editing in a different cell type. Several proteins not previously implicated in the PE intoxication pathway were identified, including GPR107, ...
The present study reports a nonviral, high-efficiency method of transfecting human umbilical cord blood-derived MSCs (hUCB-MSCs) using the new electroporation-based gene transfer technique of microporation. Microporation was utilized to introduce enhanced green fluorescent protein (EGFP) as a reporter...
A recombinant protein is one that has been cloned into a vector that supports the transcription and translation of the gene. Recombinant proteins may be expressed in a host cell and purified for biochemical studies or therapeutic purposes. Alternatively, recombinant proteins may...
tagged Gpa2p (Gα of yeast glucose receptor) with GFP after position 10 without altering the protein function and showed that Gpa2p has lipid modifications, a myristoylation site on the Gly2 and possible palmitoylation sites at cysteine residue near this myristoylation site of its N-terminus, ...
2b). First, we calculated protein–protein interaction enrichment to determine if the obtained candidate list has more or less interactions among themselves, as compared with a random set of proteins of similar size. Protein–protein interaction enrichment p value of the TMEM16K network is p <...
(i.e., non-motile cilia). InC. elegans, CFAP20 is required for the structural integrity of the inner junction and, similar to its protein neighbour PACRG (Parkin Co-Regulated Gene protein), participates in several non-motile cilia-mediated processes, including gustatory plasticity, lifespan ...