ExpressionMycoplasma hyorhinis(M. hyorhinis) is an opportunistic pathogen of pigs and has been shown to transform cell cultures, which has increased the interest of researchers. The green florescence proteins (GFP) gene ofAquorea victoria, proved to be a vital marker to identify transformed cells...
25、l. Green fluorescent p rotein as a marker forgene exp ression. Science, 1994, 263(5148) : 8025 ChalfieM. Green fluorescent p rotein. Photochem Photobiol, 1995, 62 (4) : 6516 Larrick JW, Balint RF, Youvan DC. Green fluorescent protein: untapped potential in immunotechnology. Immuno...
[2]Chalfie M , Tu Y, Euskirchen G, Ward W W, et al. Green fluorescent protein as a marker for gene expression[ J] . Science, 1994, 263: 802 -805.[3]汪恒英,周守标,常志州,马艳,秦卫华.绿色荧光蛋白(GFP)研究进展[J].生物技术,2004(03):70-72.[4]薛启汉.绿色荧光蛋白(GFP)的特性及其...
Green fluorescent p rotein - a bright idea for the study of bacterial p rotein localization. FEMSmicrobial Lett, 2001, 204 (1) : 9 [4] ChalfieM, Tu, EKivchen G, et al. Green fluorescent p rotein as a marker forgene exp ression. Science, 1994, 263(5148) : 802 [5] ChalfieM...
Green fluorescent p rotein as a marker forgene exp ression. Science, 1994, 263(5148) : 802 [5] ChalfieM. Green fluorescent p rotein. Photochem Photobiol, 1995, 62 (4) : 651 [6] Larrick JW, Balint RF, Youvan DC. Green fluorescent protein: untapped potential in immunotechnology. ...
8、 as a marker for gene expression,” science 263: 802805.19钱永健钱永健roger yonchien tsien(1952年2月1日)美国生物化学家美国国家科学院院士美国国家医学院院士美国艺术与科学院院士圣地牙哥加利福尼亚大学生物化学及化学系教授。1972年,哈佛大学化学及物理学最优等学士学位。1977年,英国剑桥大学生理学博士...
GFP from jellyfish has some important characteristics,such as there is no specificity in expression,the product it expressed without toxicity in addition to without affecting the normal growth and function of cell .As a ideal marker gene,it has been used in research of animal,plant and ...
The green fluorescent protein (GFP) from the jellyfish Aequorea victoria has recently emerged as a very powerful tool in cell biology. Fused to the protein of interest, GFP serves as a marker for gene expression as well as protein localization in living cells. We have used GFP as a marker...
O2使Tyr66脱氢氧化。6 特点 •GFP荧光极其稳定；•中度氧化剂对GFP荧光影响也不大；•灵敏度高,抗光漂白能力强；•荧光的产生不需要任何外源反应底物。7 GFP的研究历史 ——从下村修到钱永健 8 TheNobelPrizeinChemistry2008 "forthediscoveryanddevelopmentofthegreenfluorescentprotein,GFP"OsamuShimomura ...
真正意识到GFP应用前景的是Chalfie,1994年他在《科学》(Science)上发表了题为《GreenFluorescent Protein as a Marker for Gene Expression》的论文.在该文中首次报道GFP可以在细菌和线虫内发光.为标记细胞和蛋白质提供了较好的方法3。与此同时,Tsien率先提出了GFP发光的化学机制4,详细阐述了GFP是如何发光的,并于...