Cells carrying the GFP-construct show no difference in cellular survival compared to cells not carrying the construct. After certain post-irradiation growth periods cells were harvested and the numbers of GFP expressing cells and fluorescence were determined by FACS analysis. Generally, GFP fluorescence...
The green fluorescent protein (Gfp) as a tool for the analysis of gene expression in mammalian cellsThe green fluorescent protein (Gfp) as a tool for the analysis of gene expression in mammalian cellsSubramanian, SSrienc, FSubramanian et al., “The Green Fluorescent Protein (Gfp) as ...
ORFs cloned in this vector will be expressed in mammalian cells as a tagged protein with a C-terminal tGFP tag. TurboGFP (excitation/ emission max = 482/ 502 nm) is mainly intended for applications where fast appearance of bright fluorescence is crucial. It is specially recommended for cell...
文章Mammalian Expression of Infrared Fluorescent Proteins Engineered from a Bacterial Phytochrome 刊登在5月8日的Science上。 生物通报道,诺贝尔奖得主,GFP改造之父钱永健在最近的一期Science杂志上发表文章,他又合成了一种新的荧光蛋白,这使得生物学界的科学家又多了个选择。文章Mammalian Expression of Infrared Flu...
eGFP allows the use of GFP in mammalian cells. In A. Victoria, GFP plays roles as an energy transfer acceptor. It has long been used in cell and molecular biology as a reporter of gene expression. GFP can also been applied as a molecular thermometer to measure temperature accurately in ...
产品参数 质粒类型:哺乳动物表达载体 启动子:CMV 克隆方法:多克隆位点,限制性内切酶 载体大小:6598 bp 载体标签:GFP 载体抗性:氨苄青霉素(Ampicillin) 筛选标记:新霉素(Neomycin) 载体介绍 ORFs cloned in this vector will be expressed in mammalian cells as a tagged protein with a C-terminal tGFP tag. Tur...
载体宿主:大肠杆菌,哺乳动物细胞(E.coli, Mammalian cells) 细菌抗性:卡纳(kan) 真核筛选标记:新霉素(Neomycin) 5' 测序引物:EGFP-N Sequencing Primer (#6479-1) 3' 测序引物:EGFP-C Sequencing Primer (#6478-1) 载体描述:encodes an enhanced yellow-green variant of the Aequorea victoria green fluores...
Our BacMam constructs do not replicate in mammalian cells, making them Biosafety Level I (the lowest biosafety level). They can be handled with the standard precautions used for any cell-based reagents.Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center...
Our BacMam constructs do not replicate in mammalian cells, making them Biosafety Level I (the lowest biosafety level). They can be handled with the standard precautions used for any cell-based reagents.Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center...
Our BacMam constructs do not replicate in mammalian cells, making them Biosafety Level I (the lowest biosafety level). They can be handled with the standard precautions used for any cell-based reagents. Find additional tips, troubleshooting help, and resources within ourCell Analysis Support Center...