Cells carrying the GFP-construct show no difference in cellular survival compared to cells not carrying the construct. After certain post-irradiation growth periods cells were harvested and the numbers of GFP expressing cells and fluorescence were determined by FACS analysis. Generally, GFP fluorescence...
The green fluorescent protein (Gfp) as a tool for the analysis of gene expression in mammalian cellsThe green fluorescent protein (Gfp) as a tool for the analysis of gene expression in mammalian cellsSubramanian, SSrienc, FSubramanian et al., “The Green Fluorescent Protein (Gfp) as ...
文章Mammalian Expression of Infrared Fluorescent Proteins Engineered from a Bacterial Phytochrome 刊登在5月8日的Science上。 生物通报道,诺贝尔奖得主,GFP改造之父钱永健在最近的一期Science杂志上发表文章,他又合成了一种新的荧光蛋白,这使得生物学界的科学家又多了个选择。文章Mammalian Expression of Infrared Flu...
Our BacMam constructs do not replicate in mammalian cells, making them Biosafety Level I (the lowest biosafety level). They can be handled with the standard precautions used for any cell-based reagents. Find additional tips, troubleshooting help, and resources within ourCell Analysis Support Center...
types. however, cells with hematopoietic origin do not show effective transduction with celllight bacmam 2.0 reagents. find additional tips, troubleshooting help, and resources within our cell analysis support center . our bacmam constructs do not replicate in mammalian cells, making them biosafety ...
eGFP allows the use of GFP in mammalian cells. In A. Victoria, GFP plays roles as an energy transfer acceptor. It has long been used in cell and molecular biology as a reporter of gene expression. GFP can also been applied as a molecular thermometer to measure temperature accurately in ...
eGFP allows the use of GFP in mammalian cells. In A. Victoria, GFP plays roles as an energy transfer acceptor. It has long been used in cell and molecular biology as a reporter of gene expression. GFP can also been applied as a molecular thermometer to measure temperature accurately in ...
3绿色荧光蛋白GFP研究进展 万方数据
Therearetwomethodsforproductionofrecombinantproteininmammaliancells:25TransientGeneExpression(TGE)andStableGeneExpression(SGE).Incomparisonwithstablegeneexpressionwhichisacostlyandtime-consumingprocess,TGEisafastapproachforr-proteinproductionintheabsenceofselectionofhigh-expressioncellline.TGEhasbeenusedasasuitablemethod...
n rKK Mammaliancellsandits application Abstract Porcine reproductive and respiratorysyndrome(PRRS)is a contagious diseasecaused by porcine reproductive and respiratory syndromevirus(PRRSV).PRRSmainly caused reproductivedisorder,includingofpremature,abortion,fetaldeath,mummy and porcine respiratorysyndrome at variety...