Loss of estrogen receptor-alpha expression is associated with hypermethylation near its ATG start codon in gastric can- cer cell lines. Oncol Rep 2004; 11: 617-22.Woo IS,Park MJ,Choi SW,et a1.Loss of estrogen receptor--alpha expression is associated with hypermethylation near its ATG start ...
This line contains a T-DNA insertion in the second exon of the gene At1g52760, 621-bp downstream of the start codon. In this case, the border primer above, the left primer 5′-AAAACACATCAAAACGATGCC-3′, and the right primer 5′-CTCTCCTTGAATCAGCGAGTG-3′ were used for genotyping....
A genomic region downstream of the start codon (ATG) of Gpr176 was deleted. Grey box: probe used for Southern blot. (f) Southern blot of KpnI-digested DNA from Gpr176+/+, Gpr176+/− and Gpr176−/− mice. Genomic fragments from the wild-type (7.8 kb) and mutant (12.3...
According to convention [18], the adenine residue at the translational ATG start codon of the polyhedrin gene (polh) was considered to be nucleotide number 1 of the genome, and successive nucleotides were numbered in the direction of thepolhgene (see Additional file2). Analysis of the ClbiNP...
Other monocots, such as Poaceae, have the correct ATG start codon and, therefore, do not require editing at this position. The ndhD transcripts of several plants require editing at one or two additional sites (8,12). Pertinent to this work is position +59, which is C → U edited in...
A yellow fruited tomato 1 (yft1) mutant (also named n3122) was shown, by map-based cloning, to have a genetic lesion comprising a 573 bp insertion and a 13 bp deletion in the promoter region (-318 bp upstream of the ATG start codon) of an EIN2 gene, and the fruit color segregati...
5'-deletions in the EgCAD2 promoter were obtained by PCR amplification of the pOGUS-EgCAD2 plasmid containing the full-length 2.5 kb EgCAD2 promoter [51] with primers that introduced an EcoRI site at positions -301, -247, -203 and a NcoI site at the ATG start codon (additional file...
The stop codon within exon 9 is also indicated (TGA, red arrow). (b) Three mRNA variants are generated. ATG1 forms OCLN variants 1 and 2, each of these two variants has a different transcriptional start site. ATG2 generates OCLN variant 3. (c) OCLN variant 1 and 2 translate into ...
The obtained full-length cDNA was 1675 bp in length, including a 176-bp 5′ untranslated region (UTR), a 1275-bp ORF, a 3-bp stop codon (TAA), and a 221-bp 3′UTR. The transcription start site (− 176-bp upstream from ATG) was located within the 114-bp InDel (Fig. 5a). ...
5' RACE of neonatal RNA generated a single product that revealed a 95 base pair non-coding exon situated ~5 kilobases upstream of the start codon in the first protein coding exon of IGF2 (Figure 2A). In mouse and human, multiple transcripts for IGF2 have been detected with specific ...