mRNA transcriptionpri-miRNA transcriptionRNA Po IIH3K4me3Drosophila Trf4-1 (DmTrf4-1) is a polyadenylation polymerase or terminal nucleotidyl transferase (PAP/TENT) that has been reported to add poly(A) tails to snRNAs in nucleus or mRNAs in cytoplasm. Here, we found that the loss of Trf4...
The transcriptional effects on Star gene expression caused by blocking HIF1α activity resulted in suppressing the positive cAMP-mediated effects on its mRNA expression (P < 0.001), to below the levels observed in untreated/control cells (Fig. 2). In the same series of experiments, the ...
mRNA transcriptionpri-miRNA transcriptionRNA Po IIH3K4me3Drosophila Trf4-1 (DmTrf4-1) is a polyadenylation polymerase or terminal nucleotidyl transferase (PAP/TENT) that has been reported to add poly(A) tails to snRNAs in nucleus or mRNAs in cytoplasm. Here, we found that the loss of Trf4...
The transcribed mRNA was further purified using the RNeasy Mini Kit (Qiagen; 74104). To test whether Ub(G76V)-mClover3 is efficiently degraded in oocytes, 4 pl of 0.3 µM Ub(G76V)-mClover3-T2A-mScarlet mRNA was injected into oocytes collected from either 9-week-old or 65-week...
In vitro transcription/ In vitro translation Capped mRNAs were synthesized in vitro from purified PCR products using the T7 RiboMax express large-scale RNA production system supplemented with m7G cap analog (Promega). mRNAs were treated with RNase-free DNase I (Promega) and subjected to column pu...
Nonsense-mediated mRNA decay (NMD) is a mechanism by which cells recognize and degrade mRNAs that prematurely terminate translation. To date, the polarity and enzymology of NMD in mammalian cells is unknown. We show here that downregulating the Dcp2 decapping protein or the PM/Scl100 component ...
(pol) that encode proteins required for reverse transcription3. In most cases, the pol gene is preceded by an overlapping gene encoding one or more nucleocapsid proteins4, in a different reading frame. Because both coding regions are represented in a single mRNA, the question arises of how ...
For each condition, bacterial cultures were grown until final-log phase and cells were all harvested at OD 600 nm. Total RNA extraction was performed using RNeasy Mini Kit (Qiagen) with DNAse treatment (Qiagen). Enrichment for mRNA was performed using the MICROBExpress Bacterial mRNA Purification...
A recent report suggests that the S447X allele may enhance LPL mRNA translation because of reduced binding by the KH domain of AKAP1 (Ranganathan et al., 2012). Furthermore, AKAP1 transcription appears to be differentially regulated in lean and obese patients. Show abstract Gain-of-function ...
Additionally, we did not detect any alteration of the decay rate of the CAT mRNA, implying that the hnRNP K protein does not alter the half-life of mRNA. It is also possible that the transcription factors remain sequestered in an RNA-bound form, and the overexpression of an RNA-binding ...