1. 转录(Transcription):转录是指DNA序列被RNA聚合酶(RNA polymerase)复制成RNA分子的过程。在细菌中,RNA聚合酶结合到DNA的启动子区域,并沿着DNA链合成相应的mRNA分子。转录过程包括启动、延伸和终止阶段。 2. 剪接(Splicing):在某些细菌中,mRNA可能还需要经过剪接过程。剪接是指将mRNA中的内含子(intron)区域切除,保...
5' Cap保护mRNA不被外切酶降解，与3'端的poly A尾、poly A结合蛋白（PAPB）和翻译起始因子蛋白协同作用，起始蛋白质的翻译。体外转录（In Vitro Transcription，IVT）的加帽是常用的加帽方式，可以在IVT时加帽（即共转录加帽），也可以在IVT结束后加帽（即酶法加帽）【详见mRNA体外制备之酶法加帽和共转录加帽...
Non-coding mRNA structures that sense the environmental status of a cell by directly binding to small molecule ligands, such as a metabolite or an ion. This interaction changes the RNA conformation and controls gene expression through alternative splicing, transcription or translation. Peptidyl transfera...
Learn about mRNA transcription. Discover where and how DNA is transcribed into RNA in prokaryotes and eukaryotes, and examine the final product of...
5' Cap保护mRNA不被外切酶降解,与3'端的poly A尾、poly A结合蛋白(PAPB)和翻译起始因子蛋白协同作用,起始蛋白质的翻译。体外转录(In Vitro Transcription,IVT)的加帽是常用的加帽方式,可以在IVT时加帽(即共转录加帽),也可以在IVT结束后加帽(即酶法加帽)【详见mRNA体外制备之酶法加帽和共转录加帽】。
Transcription rates impacts translation, Related to Figures 3 and 4 (A) Segregation of the induced versus non-induced Rluc mRNAs on sucrose gradients. Cells expressing barcoded Rluc gene were treated for 21 hr to induce the expression of Rluc or left untreated, and subjected to BPP procedure. ...
Quantification of mRNA translation in live cells using single-molecule imaging Article 19 February 2020 System-wide analysis of RNA and protein subcellular localization dynamics Article Open access 30 November 2023 Tracking transcription–translation coupling in real time Article Open access 04 Decem...
对于体外转录(In Vitro Transcription,IVT)合成的mRNA,常见的有两种加帽方法(图2):共转录加帽和酶法加帽,具体的加帽原理与步骤【详见mRNA体外制备之酶法加帽和共转录加帽】。但是应当注意共转录加帽会出现反向加帽的结果,从而导致快速的降解和较低的翻译效率。为了避免反向加帽,抗反向帽子结构类似物(anti-reverse...
Production of mRNA was assayed indirectly using a coupled cell-free transcription–translation system in which protein synthesis is dependent on added vaccinia cores. 展开 关键词: Cell-Free System Vaccinia virus Viral Proteins RNA, Messenger RNA, Viral Ultraviolet Rays Protein Biosynthesis Transcription,...
The conversion from mRNA to protein is called transcription. Is the statement true or false? DNA polymerase is required for the transcription of DNA to produce mRNA. a. True b. False Transcription and translation can occur concurrently on the sam...