ICH Q6B guideline section 6.1.1 c statesTerminal amino acid analysis is performed to identify the nature and homogeneity of the amino- and carboxy-terminal amino acids. If the desired product is found to be heterogeneous with respect to the terminal amino acids, the relative amounts of the ...
analysis, important considerations for therapeutic drug formulation and storage. Biotherapeutic drugs can be analysed at their subunit level following reduction and/or digestion. Analysis of therapeutic antibody subunits facilitates exact molecular weigh...
molecular weight and determining glycoform heterogeneity of biopharmaceuticals.Superb spectral clarity delivers high-confidence biotherapeutic characterization to reliably quantitate and identify biotherapeutics in both research and routine laboratory environments. From intact mass analysis under denaturing and native...
and since MS analysis itself has the potential to introduce unanticipated conformational changes. “You have to take information from the MS spectra, and link them back, through orthogonal analysis methods, to solution-phase behavior.” The degree to which such correlations are possible det...
One gene can give rise to many functionally distinct proteoforms, each of which has a characteristic molecular mass. Top-down mass spectrometry enables the analysis of intact proteins and proteoforms. Here members of the Consortium for Top-Down Proteomics provide a decision tree that guides researcher...
Gas chromatography‐negative chemical ionization mass spectrometry (GC–NCIMS) has been applied to the analysis of O‐ and S‐glucuronides. The glucuronides were derivatized with pentafluorobenzyl (PFB) bromide and Tri‐Sil, and analyzed by GC–NCIMS. All glucuronides gave a large [M—PFB]?
Liquid chromatography is a valuable tool for separating these proteins prior to the MS analysis. Intact protein chromatography is most commonly used in a top-down approach in proteomics or to determine expression levels during recombinant protein synthesis. The size of the protein molecule results in...
Two techniques are primarily used for flux determination: (1) mass isotopomer analysis, and (2) extracellular metabolite balance models. Mass isotopomer analysis has been used extensively to quantitate fluxes in mammalian cells and tissues including brain, heart, and liver. In this approach, the ...
3 Georgiou, Harry M., et al., Proteomic analysis of human plasma: failure of centrifugal ultra-filtration to remove albumin and other high molecular weight proteins, Proteomics (2001), 1(12), 1503-1506. 4 Jin, Wen-Hai, et al., Human Plasma Proteome Analysis by Multidimensional Chromatography...
L Li,M Whiteman,PK Moore - 《Journal of Cellular & Molecular Medicine》 被引量: 116发表: 2009年 Capillary electrophoresis‐mass spectrometry for the analysis of intact proteins 2010–2012 Since its introduction in 1987, CE‐MS has become an increasingly important technique for the analysis of ...