(1:25 dilution; Abcam), and CD45 (1:25 dilution; Biolegend) antibodies. DRAQ7 (1:10 dilution; Biostatus) far-red fluorescing viability staining was used to exclude dead cells. Cells were analyzed by flow cytometry on a Gallios cytometer (Beckman Coulter, Indianapolis, Indiana, USA) using...
Accordingly, the present invention provides a compound that inhibits the interaction of an IGF with any one of its IGFBPs and does not bind to a human IGF receptor, excluding (1-27,gly4, 38-70)-hIGF-I, excluding antibodies against an IGFBP that do not bind to a human IGF receptor, ...
T1D is an autoimmune disease with the presence of autoantibodies, of which the islet cell antibodies (ICAs) have been found to react against the cytoplasmic proteins in the β cells. Therefore, insulin gene expression in extrapancreatic sites is the logical step to begin with. The initial gene...
However these surfactants caused irritation and could lead to anti-insulin antibodies. The advantage of pulmonary aerosol is that significant absorption of insulin takes place without the use of absorption promoting agents. The long term effects of this delivery has to be assessed. Besides these the...
Anti-GRP78 (BiP) and anti-GAPDH antibodies were from Abcam (Cambridge, MA). Monoclonal anti-CCAAT-enhancer-binding homologous protein (CHOP) antibody was from Thermo Fisher Scientific (Waltham, MA). Human liver tissues were obtained from the National Institutes of Health (NIH)-sponsored Liver ...
The β-actin antibodies as staining used as a control to ensure equal protein loading in each lane of the gel. The membrane was washed three times each for 5 min in TBST, and then shaken in a solution of anti- mouse IgG or anti-rabbit IgG secondary antibodies. After repeating the ...
The cells were then cultured for 12 h in GM or AIM followed by ChIP analyses using anti-H3K9me2 and anti-C/EBPb antibodies. Precipitated DNA samples were analyzed by q-PCR with primers covering -360/-155 region of the PPARγ2 promoter. Mean + SD, n = 3. *p < 0.05. ...
We used specific antibodies targeted against a (15), e (15), bII (23), and d (19) PKC isoforms. These isoforms are expressed in the brain (31–34) and activated by an increase in DAG in the cytosol or by a phorbol ester such as PMA (35). They are inhibited by calphostin C,...
Each membrane was incubated with the primary antibodies de- scribed above. Blots were developed with a 1:1000 dilution of the HRP-conjugated secondary antibody (Cell Signaling). Proteins were visualized using Immobilon Western HRP Reagent (Millipore, Billerica, MA, USA). siRNA. PC-3 cells were...
The bound antibodies were detected by horseradish peroxidase-labeled secondary antibody (1:1000 dilution). Protein bands were visualized using an enhanced chemiluminescence detection system (ECL) according to the instruction given by the manufacturer. The experiments were performed in triplicate. 2.7. Two...