Markedly increased serum insulin concentration (i.e., >400 µU/ml) in blood obtained more than 24 hours after the last insulin injection in an insulin-treated diabetic suggests insulin-binding antibodies. Serum insulin concentration is typically less than 50 µU/ml 24 hours after the ...
anti-insulin antibodiesdeterminationSPR based immune biosensorIt was developed the immune biosensor for the determination of the anti-insulin level in the blood of patients with diabetes. The next questions were under the detailed consideration: ways of the optimal treatment of the transducer surface ...
Antibodies Rabbit polyclonal anti-DDR2, IgG Invitrogen/ ThermoFisher Cat# PA5-27752; RRID: AB_2545228 Rabbit polyclonal anti-HSL (LIPE), IgG Cell Signaling technology Cat# 4107; RRID: AB_2296900 Mouse monoclonal anti-CD36, IgA Abcam Cat# ab23680; RRID: AB_447608 Rabbit polyclonal anti-CSD...
Evidence for altered transport of insulin across the blood-brain barrier in insulin-resistant humans. Acta Diabetol. 51, 679–681 (2014). Article CAS PubMed Google Scholar Sartorius, T. et al. The brain response to peripheral insulin declines with age: a contribution of the blood-brain ...
Gamble (1975) "Islet-Cell Antibodies in Juvenile Diabetes Mellitus of Recent Onset" The Lancet 1:880-882. Maclaren, N.K., S.-W. Huang, J. Fogh (1975) "Antibody to Cultured Human Insulinoma Cells in Insulin-Depdnent Diabetes" The Lancet 1:997-1000. Kaufman, D.L. et al. (1992)...
enrolled relatives who were positive for islet cell antibodies by immunofluorescence and positive for insulin autoantibodies by radioimmunoassay, had first-phase insulin release on intravenous glucose tolerance test higher than threshold (defined below), and had a normal oral glucose tolerance test result....
In a phase 3 type 1 diabetes clinical trial of NOVOLOG, initial increase in titers of antibodies to insulin, followed by a decrease to baseline values, was observed in regular human insulin and insulin aspart treatment groups with similar incidences. These antibodies did not cause deterioration ...
Blood glucose was measured directly using a glucometer (Contour, Bayer). Plasma or serum clinical biochemistry test was performed by the Department of Clinical Chemistry at the University of Cologne Hospital; insulin and C-peptide were quantified by enzyme-linked immunosorbent assay (ELISA) kits (Cr...
The earliest insulins were derived from animal pancreas. These extracts had significant variability in their onset, peak, and duration of action depending on purification techniques. They also had a propensity to induce anti-insulin antibodies24and have since been phased out in the United States. ...
A serum glucose concentration orurinalysisshould be obtained to determine circulating concentration of glucose. Anti-insulin antibodies should also be evaluated. Drug Effects on Levels Hyperinsulinemia: Alcohol,monoamine oxidase(MAO) inhibitors, beta-blockers likepropranolol,salicylateslikeaspirin, andanabolic ...