The theoretical limit of detection for each protein using the BD CBA Human Inflammatory Cytokines Kit is defined as the corresponding concentration at two standard deviations above the median fluorescence of 20 replicates of the negative control (0 pg/ml). Cytokine IL-8 IL-1β IL-6 IL-10 TNF...
TNF is a crucial cytokine involved in immunity and inflammation, and plays a key role in driving chronic inflammation in a number of inflammatory and autoimmune diseases8,9. TNF stimulates bone erosion in many inflammatory diseases, such as in RA3,5,10. TNF blockade therapy has demonstrated the...
TNF-α and IL-1β are the key components of cytokine network and the important inflammation mediators of ALI initiation, which mainly secreted by mononuclear macrophages53,54. To further address the molecular mechanism underlying PRKCA-mediated EGCG protection against LPS-mediated ALI, we examined ...
2e). All of those confirmed that BP lesions were characterized by a type 2 immune environment, with the dramatic enrichment of Th2 cells and type 2 chemokine-expressing DC cells, and the increase trend of M2 macrophage (CD163+ Macro) and type 2 cytokine-expressing Proliferating T cells. ...
To measure the effects of HCE-EA on pro-inflammatory cytokine and inflammatory mediator’s expression in RAW 264.7 cells, we used the following methods: cell viability assay, Griess reagent assay, enzyme-linked immunosorbent assay, real-time polymerase chain reaction and western blotting analysis. Re...
Pahumunto N, Chotjumlong P, Makeudom A, Krisanaprakornkit S, Dahlen G, Teanpaisan R. Pro-inflammatory cytokine responses in human gingival epithelial cells after stimulation with cell wall extract of Aggregatibacter actinomycetemcomitans subtypes. Anaerobe. 2017; 48: 103-9....
by both TPA-treated THP-1 cells and human monocyte-derived macrophages. This enhanced response to the presence of apoptotic cells also results in the suppression of the production of the pro-inflammatory cytokine IL-8. In addition, human IgG and FcγRI appear to be critical in triggering the...
et al. Growth factor and pro-inflammatory cytokine contents in platelet-rich plasma (PRP), plasma rich in growth factors (PRGF), advanced platelet-rich fibrin (A-PRF), and concentrated growth factors (CGF). Int J Implant Dent 2, 19 (2016). https://doi.org/10.1186/s40729-016-0052-4 ...
Interleukin-10 (IL-10) is a key anti-inflammatory cytokine responsible for intestinal immune homeostasis, mainly through the inhibition of pro-inflammatory cytokines17. When the intestinal immune homeostasis is disturbed by stress, macrophages and T lymphocytes are activated to release pro-inflammatory ...
ELISA For cytokine secretion analysis, human or murine macrophages were seeded and polarized in 24-well plates at 150,000 cells/well. For N, chH, cyH incubation, 750 µl of CO2 independent medium + 4 mM L-glutamine + 3.75 g/l D-glucose were added per well. For murine...