We ran all standards and participant samples in triplicate (single analyte kits require a smaller volume of serum for analysis than multiplex kits). We performed all experiments as per the manufacturer’s instructions and analysed the plates on a MESO QuickPlex SQ 120 instrument. Numeric results ...
MAP Fluorokine cytokine kits were purchased from R&D (Oxon, UK). The procedures closely followed the manufacturer's instructions. The analysis was conducted using a conventional flow cytometer (FC500 MPL, BeckmanCoulter, Nyon, Switzerland). Toxicology studies in cynomolgus monkey Preclinical toxicology...
The results of assays on the supernatants from 6-day ex vivo cultures of PBMCs for levels of IL-2, IL-9, and IL-15 using ELISA kits are shown in supplemental Figure 2. The 6-day ex vivo spontaneous proliferation of ATL and HAM/TSP PBMCs was associated with secretion into culture medi...
Currently, kits for human, mouse, rat, canine, monkey and porcine samples are available to cover various model organisms, with permanently growing number of covered analytes and species. Moreover, custom assays can be built by antibody coupling to magnetic beads and Luminex Corp. provides support...
Levels of interferon-γ (IFNγ), interleukin-2 (IL-2), IL-4 and IL-10 that were spontaneously produced/released by the splenocytes were then measured using ELISA kits, following the manufacturer protocols for each kit (R&D Systems, Minneapolis, MN, USA). The final OD value for each ...