( 7 ) and dissolvedin isopropanol.Plasma levels of interleukin-6 (IL-6) and solubleintercellular adhesion molecule-1 (sICAM-1) were mea-sured with mouse sandwich enzyme-linked immuno-sorbent assay (ELISA) kits for cytokine (R&D SystemsInc., Minneapolis, MN, USA). Tumor necrosis factor- ?
ELISA kits for human or mouse IL-1β, IL-6 and TNF-α were purchased from Anogen (Beijing, China). An ELISA kit for human HIF-1α was purchased from Sigma–Aldrich (St. Louis, MO, USA). Mouse IFN-α and IFN-β ELISA kits were purchased from Neobioscience. Serum, alveolar lavage ...
Cytokine production of infected PBMCs were measured in cell-free supernatants using Human ELISA Ready-Set-Go! kits for four cytokines, i.e. IL-4, IL-8, IL-13, and MCP-3 (all from eBioscience, Affymetrix, Santa Clara, CA, USA) according to manufacturer’ instructions. Calculated minimum ...
eHMGB1 was detected in these same lung homogenates by ELISA. *p < 0.007 (Student's t test). high yield that is suitable for scale-up. The good bioavailability, high water solubility, lack of toxicity, and selective TLR4 activity make FP7 an ideal compound to be developed as a...
Interleukin levels were determined using highly sensitive and specific enzyme-linked immunosorbent assay (ELISA) kits based on the principle of the double-antibody sandwich technology for chicken IL-1β and chicken IL-8 (Abbexa-Ltd®, Cambridge, UK). The optical density (OD) was measured spectro...
In cytokine analysis, the commercial ELISA ready-to-use kits with optimized antibody pairs are available for broad spectrum of targets and species. ELISA immunoassays are quite popular because of their high specificity, sensitivity, rapid turnaround time, convenience, the ease of performance and a ...
These results pave the way for further clinical studies to validate the efficacy and safety of CBD-X extract as a potential treatment for asthma and other chronic inflammatory lung diseases. 4. Materials and Methods 4.1. Reagents ELISA kits for human IL-5, IL-13, IL-8, and IL-6 or mouse...