IFN-γ and Granzyme-B (GrB) in cell culture supernatants were quantified using Human IFN-γ (DY285B) or GrB (DY2906) DuoSet ELISA kits from R&D Systems, following the instructions of the company. Cytokines including TNF-α, IL-1β, IL-12p70 and IL-18 were measured by Luminex Multiplex...
There is no information on cytokine profiles for use as markers of protection in Campylobacter jejuni infection. To study this, we used outer membrane protein (MOMP [PorA]) as the vaccine for protection and spleen cell cytokines as markers of protection.
ELISA The levels of M-CSF (MMC00B, R&D Systems) and C-terminal telopeptide of type I collagen (CTX-1; AC-06F1, Immunodiagnosticsystems) in the cell culture supernatants were quantified using their respective enzyme-linked immunosorbent assay (ELISA) kits, according to the protocols provided by...
Levels of proinflammatory cytokines TNF and MCP-1 for the monocytes and macrophages were quantified using specific ELISA kits (R&D, NJ, USA) according to the manufacturer’s recommendations. Data were processed using Milliplex Analyst, and significance was calculated using the one-way analysis of ...
Soluble and insoluble Aβ40 and Aβ42 levels were quantified by colorimetric ELISA kits (Wako, Japan) as previously described with minor modifications [17]. Somatosensory cortex (5–10 mg) was homogenized in 50 μl of lysis buffer (Pierce™ IP Lysis Buffer, cod. Cat 87787 Thermo Scie...
Cytokine ELISA Introduction Due to the amplifying potential of enzyme labels, immunoassays which utilize enzyme-conjugated antibodies have become increasingly popular because of their high specificity and sensitivity.1 In 1971, Engvall and Perlmann2 coined the term “enzyme-linked immunosorbent assay” ...
ELISA kits for human or mouse IL-1β, IL-6 and TNF-α were purchased from Anogen (Beijing, China). An ELISA kit for human HIF-1α was purchased from Sigma–Aldrich (St. Louis, MO, USA). Mouse IFN-α and IFN-β ELISA kits were purchased from Neobioscience. Serum, alveolar lavage ...
Enzyme-linked immunosorbent assay (ELISA) MH7A cells with various transfections were maintained in 24-well plates for 48 h. The cell supernatant was harvested and TNF-α and IL-1β levels in the supernatant were analyzed using commercial kits (#PT518 and #PI305; Beyotime, Shanghai, China) ...
Nrf2 (NF-E2-related factor-2) transcription factor regulates oxidative/xenobiotic stress response and also represses inflammation. However, the mechanisms how Nrf2 alleviates inflammation are still unclear. Here, we demonstrate that Nrf2 interferes with
ELISAs were performed with DuoSet ELISA Kits (R&D Systems, Minneapolis, MN, USA) according to manufacturer’s instructions, for which FLS cell culture supernatants were used undiluted. The optical density at 450 nm, with a wavelength correction set to 540 nm, was evaluated using the Spectra...