in-house neutralization assayBackground & AimHepatitis B virus-e-antigen (HBeAg) is an affordable viral marker to assess viral replication kinetics and response to antiviral therapy. In the absence of confirmatory assays, discrepant or false-positive HBeAg results are resolved by screening for other...
performance of the ELISA was highly imbalanced, with 96% sensitivity at the expense of 22% specificity. As for the clinical performance, the negative predictive value reached 87% while the positive predictive value was 51%. Our results stress the need for highly specific and sensitive assays and...
This study aimed to evaluate the correlation between in-house and commercial binding-specific IgG antibodies and between in-house and commercial SARS-CoV-2 surrogate virus neutralization tests (sVNT). Samples from healthcare workers who received vaccines against SARS-CoV-2 were tested for RBD-...
Along the extruder, a sequential addition of NaOH (3.75% (w/v)), heating at 150 °C, and neutralization with H2SO4 (0.5% (w/v)) was performed. In Module 9, a stainless-steel filter removed the liquid part from the solids. In Module 10, there were two reverse screws that acted ...
this is the first in-house ELISA that is evaluated against micro-neutralization (MN) assay (the gold standard to assess the presence of virus-specific antibodies and measure their neutralization). In addition to its potential diagnostic application, the availability of this assay could be valuable ...
Delta and Omicron. Dashed lines on x-axis represent the positive cut-off value (OD > 0.26) for in-house CovIgM-ELISA and the dashed line on the y-axis represent the positive cut-off (sVNT%≥≥30%) for the neutralization assay. There was significant correlation (*p= 0.02225) between sV...