MAXQDA Tip of the month: In-vivo coding In-vivo coding: What does this mean? The term in-vivo comes from grounded theory research and means that words or terms used by the interviewees are so remarkable that they should be taken as codes. In-vivo coding adds these terms of the ...
To monitor tissue-wide clonal expansion using an in vivo single-cell CRISPR strategy, we adjusted the CRISPR droplet sequencing (CROP-seq) system5,6by substituting the puromycin cassette with mCherry to enable selection of infected cells by fluorescence-activated cell sorting (FACS) (Fig.1a). We...
Fig. 1: circRNAs can be specifically downregulated by genetically encoded shRNA in vivo. aScheme of the shRNA strategy to knockdown circRNAs in vivo. ShRNAs are generated from a UAS-based miR-1 like precursor (see Materials and methods).bRelative levels of the targeted circRNAs in fly heads ...
Triple negative breast cancer (TNBC) patients exhibit poor survival outcomes and lack effective targeted therapies. Using unbiased in vivo genome-wide CRISPR screening, we interrogated cancer vulnerabilities in TNBC and identified an interplay betwe
As no single delivery approach is likely to be ideal for all possible applications, we note advantages and disadvantages of each strategy and highlight opportunities for further development. Therapeutic gene editing strategies Several types of gene editing agents have been used for in vivo gene ...
Faculty Opinions recommendation of Sensitivity to perturbations in vivo implies high noise and suggests rate coding in cortex. M Mehta,A Kumar 被引量: 0发表: 2010年 Transient and Persistent Dendritic Spines in the Neo cortex In Vivo We have compared these techniques and fi nd that each has ...
As mentioned previously, the most robust strategy to obtain a more comprehensive multi-omics profile is to process continuous tissue sections, in which each section is queried by different omics. However, continuous tissue section sampling might give rise to sample heterogeneity deviation. Researchers ...
while cell membrane penetration and peptide stability in vivo were considered to be potential limits for its clinical applications [27,28]. Thus, there is still a lack of effective target drugs for the Hippo/YAP axis in human cancer. Based on this, we shifted our strategy to inhibiting the ...
g, Ex vivo glioma-associated microglia T cell-suppression assay. Top, CD4+ T cells after co-culture with microglia. n = 10 individual microglia samples isolated from IDH-WT tumor-bearing mice, n = 9 individual microglia samples isolated from IDH-mutant tumor-bearing mice. Bottom, ...
These results suggest that in vivo self-assembled siRNA is a promising combination therapeutic strategy for UC with an acceptable safety profile. However, the animal models were maintained under specific pathogen-free conditions in this study, and there is a certain risk that the multi-targeted ...