In vivo coding is a form of qualitative data analysis that places emphasis on the actual spoken words of the participants. This form of coding can be especially helpful when researchers interact with participants from a particular culture or microculture to help highlight how those participants use...
MAXQDA Tip of the month: In-vivo coding In-vivo coding: What does this mean? The term in-vivo comes from grounded theory research and means that words or terms used by the interviewees are so remarkable that they should be taken as codes. In-vivo coding adds these terms of the ...
circulation for longer and was ultra-small, which increased its ability to penetrate the blood–brain tumour barrier. We then investigated ASO-based cancer therapy using the brain tumour model. In the present study, a long non-coding RNA,TUG1, was selected as a target molecule becauseTUG1silen...
Together, the top-down and bottom-up in vitro SCRaMbLE systems provide an efficient strategy to generate rearranged and optimized genetic structures. We have demonstrated that in vitro SCRaMbLE has several advantages over the in vivo method. 1) In vitro SCRaMbLE is highly controllable as compared ...
cDNAs for bichir Polypterus ornatipinnis, sturgeon Acipenser baerii and caecilian Typhlonectes natans were sequenced using degenerated primers designed based on amino-acid alignments of previously known sequences and the rapid amplification of cDNA end (RACE) method to obtain complete coding sequences...
S1B). Twenty eight nucleotides upstream of the clustered regularly interspaced short palindromic repeats (CRISPR) associated protein 9 (Cas9) cut site (3–4 bp upstream of the PAM: TGG) were deleted in the coding region of Sspka2, and the intervening sequence contains plasmid vector sequences...
Site-directed mutations of T152, T210 or both by glutamic acid were made in the coding region of Rv1747_FHA domain, to emulate different phosphorylation states of the domain. Three mutants of the FHA domain, FHA_T152E, FHA_T210E and FHA_T152E/T210E (double mutant), were produced. The...
(B) illustrate potential coding sequences that are probably untranslated because of stop codons. The tilde denotes a shift in the open reading frame. The presumably longest predicted ORF are shown as black bars under the protein domain depiction. Species abbreviations: hs,Homo sapiens; pt,Pan ...
first in-vivo application of single-cell multicolour RGB marking to the study of mature neuronal populations and the temporal and spatial dynamics of neurogenesis at the subventricular zone and the dentate gyrus, providing the basis for a broadly applic- able method to track and manipulate CNS ...
In the MOB, the number of BrdU-positive nuclei was established by counting peroxidase/DAB-stained nuclei in three representative OB coronal (anterior, medial and posterior) or parasagittal (from lateral to medial) sections per animal using a systematic random sampling method. Newborn cells were qua...