The DNA methylation analysis of cells with a Foxp3hiTreg phenotype in this early phase of Treg induction showed a clear dynamic in Foxp3 CNS2 methylation, which are only partially captured by the in vitro differentiation system. Thus, initial Treg induction from naive CD4+T cells causes rapid ...
these conditions, instead of inducing the activation and clonal expansion of T cells, tolDCs promote T cell hyporesponsiveness that is mainly mediated by the induction of T cell anergy, T cell depletion triggered by apoptosis induction or the induction of Treg differentiation (reviewed in58,59). ...
An R.roseainduced upregulation of hypoxiainducible factor1α (HIF1α) contributed to the suppression of Treg differentiationinvitro. Differences in the effect of R.roseainvitro andinvivo were also observed using a mouse model of microgravity. The results of the current study suggest that R....
顺序免疫策略,首先是肌肉注射,然后是气管内加强注射,通过高水平的 IgA 和组织驻留记忆 T 细胞反应显著增强粘膜免疫,从而建立起对假病毒感染的强大防御。 10.Proteomic profiling of human plasma extracellular vesicles identifies PF4 and C1R as novel biomarker in sarcopenia. 人类血浆细胞外囊泡的蛋白质组学分析表...
A unique CD8+ splenic DC subset, which expresses DEC205, a type I transmembrane protein with multiple C-type lectin domains, has been identified in the mouse spleen, and preferentially drives differentiation of iTregs[65]. CD8+DEC205+ DCs can drive iTreg differentiation both in vitro and in...
An ideal scaffold should provide an appropriate environment for cell adhesion, proliferation/differentiation, and migration to allow the generation of in vitro tumor models that closely recapitulate essential cell–ECM interactions. In this context, tumor cells can be cultured within biomaterials, including...
In our in vitro experiments, we found curcumin could promote Treg differentiation and increase the production of IL-10. Conclusions Curcumin can reduce the degree of severity of ALI and uncontrolled inflammation through promoting the differentiation of naïve CD4 + T cells to CD4+ CD25+ FOXP3+ ...
Embryonic stem cells, derived from the inner cell mass of murine blastocysts, can be maintained in a totipotent state in vitro. In appropriate conditions e... DANI,C. - 《Journal of Cell Science》 被引量: 1239发表: 1997年 Neoplastic differentiation: Characteristics of cell lines derived from ...
In vitro T cell differentiation assay Naïve CD4+ T (CD4+CD44low) cells from spleens of C57BL/6 mice were sorted, and activated with plate-bound 2 μg/ml anti-CD3 (145-2C11; BioLegend) and 1 μg/ml anti-CD28 (37.51; BioLegend) antibodies. The cells were treated with variou...
Human FOXP3+ regulatory T (Treg) cells are central to immune tolerance. However, their heterogeneity and differentiation remain incompletely understood. Here we use single-cell RNA and T cell receptor sequencing to resolve Treg cells from healthy individ