In Vitro Treg Suppression AssaysTreg, In vitro, Suppression, Foxp3Determining the activity of a regulatory T-cell population in vitro is often the first step in analyzing its function. To obtain reliable and reproducible results, it is critical to follow the protocol that is most applicable to ...
Here we present our adapted protocol for assaying regulatory T cell suppression of Celltrace Violet-labeled responder T cells. 展开 关键词: Treg 调节性T细胞 Proliferation 增殖 Suppression 抑制 Regulatory t cell 调节T 细胞 Flow cytometry 流量细胞计数法 ...
Regulatory T (Treg) cells are a highly immunosuppressive population of CD4+T cells characterized by the expression of the transcription factor forkhead box protein P3 (FOXP3). Tregcells control immune responses and maintain peripheral tolerance1,2,3,4. Tregcells have been suggested to be heterogene...
e, In vitro suppression assay with CD25hi Treg cells immunomagnetically purified from spleens of naive wild-type or CD28-knockout mice cultured for 48 h with CellTraceViolet-labelled CD8+ T cells and B cells and activated with anti-CD3 in the presence of anti-CTLA-4 or IgG control and ...
Also, the masking of MHC class I molecules on MDSCs abrogated immune suppression by MDSCs.6 However, MHC Cl I−/− MDSCs arising from tumors grown in β2-microglobulin–deficient mice did not exhibit any impairment in inhibiting T-cell proliferation in vitro.7 Because transplantation of ...
To validate the immunosuppressive function of the Tregs induced by apCAFs (iTregs), we performed a Treg suppression assay. First, we co-cultured naive OT-II CD4+ T cells with apCAFs in the absence or presence of OVA peptide. The uninduced CD4+ T cells (without OVA) and iTregs (with ...
2.4. In Vitro Antigen-Specific Suppression Assay To incite an immune response to an insulin-relevant antigen, we gave the insulin peptide B9-23 emulsified in an equal amount with complete Freund adjuvant to young nondiabetic NOD mice. At ten days following antigen challenge, mice were humanely ...
In CSCC, triggering OX40 with an agonist antibody overcame the suppression exerted by Treg, increasing T-cell effector proliferation in vitro [162]. However, when the agonist BMS-986178 has been evaluated in patients with advanced cancer in monotherapy or in combination with nivolumab and/or ...
IL-27 and IL-35 are anti-inflammatory cytokines that can regulate Treg responses [148,149]. IL-35 has been shown to play a key role in FoxP3+ Treg suppression and in the induction of iTr35 [80,149]. Treatment with IL-35 has shown to lead to EAE amelioration with concomitant induction...
Bioinformatics analysis was performed using TCGA data from HNSC and CESC to screen for METTL3, classify patients into METTL3 high or METTL3 low status based on their expression, and perform enrichment analysis as well as in vitro and in vivo experiments for HPV correlation and immune response. ...