Here we present our adapted protocol for assaying regulatory T cell suppression of Celltrace Violet-labeled responder T cells. 展开 关键词: Treg 调节性T细胞 Proliferation 增殖 Suppression 抑制 Regulatory t cell 调节T 细胞 Flow cytometry 流量细胞计数法 ...
The Treg suppression test is a frequently used assay for determining the regulatory T cells' (Treg) capacity to inhibit T cell growth.Example (a) Representative flow cytometry plots of CD4+ LN T cells from B3K506TCR Tg Rag−/− (B3K506 Tconv) and B3K506 TCR Tg Foxp3 Tg Rag−/...
To obtain reliable and reproducible results, it is critical to follow the protocol that is most applicable to your experimental question. We have outlined below a basic in vitro suppression assay as well as a variety of alternative/additional protocols that can be utilized alone or in combination...
Therefore, we tested the ability of Tregs lacking MAVS to suppress proliferation of conventional T cells using a CFSE-based suppression assay. Following three days of co-culture, WT and Mavs−/− Tregs were equally proficient in suppressing T cell proliferation (Fig. 3b), suggesting that ...
was regulating the suppressive function of nTreg cells, a suppression assay was performed using WT and SMYD3 À / À FACS-sorted CD4 þ CD25HI cells (Foxp3 þ nTreg cells; 495% purity) isolated from na¨ıve mice. Our data show that both WT- and SMYD3 À / À -...
6C-D). We re-performed cytotoxicity assay of CD8+ T cells with an additional shRNA targeting Lkb1, and data uncovered that knockdown of Lkb1 in Tregs resulted in decreased suppression of the cytotoxic activity against SU-DHL4 cells compared to the that of the control group (Additional file ...
It has been reported that administration of exosomes derived from DC/IL-4 was able to modulate the activity of APCs and T cells in vivo through a MHC class II and partly FasL/Fas-dependent mechanism, resulting in effective treatment of CIA and suppression of the DTH response [36]. In our...
Author links open overlay panelJorg van Loosdregt 1, Yvonne Vercoulen 2, Teun Guichelaar 3, Yoony Y.J. Gent 4, Jeffrey M. Beekman 1, 2, Olivier van Beekum 5, Arjan B. Brenkman 5, Dirk-Jan Hijnen 4, Tuna Mutis 3, Eric Kalkhoven 5, Berent J. Prakken 2, Paul J. Coffer 1...
This observation suggests that monkey CCR4+ Tregs may directly suppress the maturation of monocyte similar to the previously reported effect of CCR4+ Tregs on suppression of dendritic cell (DC) maturation (Bayry et al., 2008). Further characterization of the effects on monocyte and DC using this...
As the Tregs were placed under the kidney capsule with the islets in these experiments (Fig. 1a), this left several possible reasons for the defective protection afforded by Lta À / À nTreg: Lta À / À nTreg could be defective in suppression; have a proliferative or survival ...