IQVIA Laboratories offers a suite of in vitro assays to support drug-drug interaction testing, including cytochrome P450 (CYP) enzyme inhibition (IC50), time-dependent CYP inhibition, induction of CYP enzymes, and CYP reaction phenotyping. All in vitro metabolism assay results are presented in fully...
Figure 1— Example of suspension metabolism study design using a 12-well format. Determine the in vitro half-life (t1/2) of the parent compound by regression analysis of the percent parent disappearance vs. time curve. Intrinsic clearance in vitro (Clint in vitro) ca...
The mTOR pathway plays a central role in regulation of cell growth, metabolism, and nutrient sensing. Its complexity and essentiality are further emphasized by its multiple regulatory complexes and negative feedback signaling loops. PIK3CA activating mutations lead to hyperactive signaling downstream of ...
In vitro methods may also have utility for predicting important pharmacokinetic parameters, such as oral absorption and hepatic metabolism. For example, oral bioavailability can be predicted by measuring drug transport across a monolayer of Caco-2 human colon carcinoma cells, as discussed in Chapt...
Cell vitality assay The in vitro assessment of the enzyme-inhibitory capacity of CA-SF against CAIX was performed by evaluating cell vitality by means of the MTT assay. The observed activity of CA-SF was compared with acetazolamide (AZ), a clinically approved CA inhibitor9,44. The experiments...
The 4 non-carcinogens found positive in the Ames test and negative in the in vitro mammalian cell assays were: C.I. Food Red 3 is an azo dye, and requires reductive metabolism to exert its genotoxic effects. For the other 3 substances, the mammalian cell studies do not meet current ...
[59]. It is conceivable that the seed oil trait is closely related to plant metabolism. Indeed, the composition of the bacterial community clearly changed when the soils were treated with the root exudates of the soybean cultivars whose seed quality varied (Fig.2b). However, among the above ...
In vitro tumor experiments showed the significant modulation of intracellular carbohydrate, amino acid, and lipid metabolism after treatment with SS NPs, suggesting that SS NPs may achieve antitumor effects through the mitochondrial apoptotic pathway and induction of metabolic reprogramming in tumor cells....
多种酶体系用于肽类药物体外代谢性质的研究 in vitro metabolisms of peptide drugs in various enzymatic systems.pdf 2015-02-14上传 多种酶体系用于肽类药物体外代谢性质的研究 in vitro metabolisms of peptide drugs in various enzymatic systems 文档格式: ...
At level 1 of KEGG, the cellular processing pathway was significantly decreased between the GMM and non-cultured MIX inoculum (Fig.6e). On a deeper resolution, including level 2 KEGG, data showed the highest significant decrease of the cell motility, transcription, and lipid metabolism in GMM ...