Biochemical assays are mainly based on a combination of electrophoretic and hybridization techniques. Hybridization techniques are well-characterized, sensitive, and quantitative. Polymerase chain reaction, fluorescence in situ hybridization, and micro-arrays are examples of highly sensitive hybridization ...
Efflux transporters facilitate the export of compounds from the cell, both in some cellular assays, also in ADME studies. These transporters belong to the ATP-binding cassette (ABC) family. P-glycoprotein (Pgp) is one of the most widely known efflux transporter which has a great effect on the...
Cell line studies performed in vitro also provide the opportunity to study the biochemical and molecular effects of a new agent on various cell processes, such as macromolecule synthesis of RNA, DNA, or proteins. Effects on tumor cell growth, cell cycle distribution, cell differentiation, or...
For more than 30 years, Cisbio has been serving the needs of the clinical and drug discovery communities, delivering a world-class portfolio of assays designed to help find answers faster. We're passionate about what we do. And we firmly believe "interac
Combined activity of endolysin ElyA1 and colistin inin vitroassays As ElyA1 is only active when the outer membrane of the target bacterial cell is solubilized, the MIC of the endolysin could not be determined using the microdilution checkerboard test. We therefore aimed to detect any decrease ...
In Vitro ADME Assays In Vitro ADME assay is an essential step in drug development. We will help you to understand the Adsorption, Distribution, Metabolism, Excretion (ADME) as well as the Pharmacokinetics (PK) of your compounds, which will help both your
In order to demonstrate the biological activity of these two compounds for human cancer cells,in vitroantiproliferative activities assays against the human cancer cell lines Hela229 were carried out subsequently. Cellular morphological changes were observed under IPCM, and the nucleus morphological characte...
3. In Vitro and In Vivo Assaysin vitro assaysin vivo assaysinhibitory potencyallosteric inhibitorscell assaysPublication » In vitro and in vivo assays.doi:10.1002/9781118354483.ch3Adam R. JohnsonWiley-Blackwell
While functional effects such as the ion-channel assays are important, adverse structural effects can also be critically detrimental. A recent in vitro assay using cardiomyocytes derived from stem cells is claimed to be capable of assessing both structural and functional damage in the same assay, pr...
In vitrodigestibility is the simulation of protein digestion in test tube assays. It is used for the quantification of digestibility, the extent of protein hydrolysis in both mono and polygastric animals including humans and ruminants, and now fish and crustaceans. Although these assays do not exac...