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Understanding the common dimension of mental disorders (such as anxiety, depression, and drug addiction) might contribute to the construction of biological frameworks (Research Domain Criteria, RDoC) for novel ways of treatment. One common dimension at t
The PCR reaction were performed in 25 μL mixture containing 2.5 μL of 10× Buffer (with Mg2+), 0.5 μL of each universal primers, 1 μL of dNTP (2.5 mM), 15 μL of sterilized water, and 0.5 μL of genomic DNA. The PCR products were checked for the expected size on 1.0% ...
Rats were exposed to five footshocks paired with social isolation. One week after trauma but before extinction, animals were tested in the Open Field and Social Interaction tasks for the identification of a predictive variable to identify susceptible and resilient animals before the possible appearance...
The combination of oils with different degree of saturation contributes to improve the utilization efficiency of the mixed oils and may reduce the cost of oil supplemented. An experiment was conducted to evaluate the effects of oils with different degree of saturation on the fat digestibility and ...
Next, transcriptomic assays were combined with network pharmacology to identify the potential differentially expressed mRNAs. Immunofluorescence staining, real-time polymerase chain reaction, western blotting, and transmission electron microscopy were performed to elucidate the underlying mechanisms. Results BYHWD...
All studies were carried out in accordance with the National Institutes of Health Guide for the Care and Use of Laboratory Animals. 2.3. Study design The first series of experiments was to identify endogenous substrates for Oatps and Cyp3a in rats. In the first experiment, rats were divided ...
For the final reaction, 5 μL qPCR mix was added in each well together with 1 μL diluted cDNA (in duplicate, with water and RT-controls included on every 384-well plate). Comparative Ct measurements and melting curves were quantified using QuantStudio 6 flex qPCR device (Life technologies)...
Postoperative cognitive dysfunction (POCD) affects the outcome of millions of patients each year. Aging is a risk factor for POCD. Here, we showed that surgery induced learning and memory dysfunction in adult mice. Transplantation of feces from surgery m
Short-term memory deficits have been associated with prefrontal cortex (PFC) dysfunction in Alzheimer’s disease (AD) and AD mouse models. Extratelencephalic projection (ET) neurons in the PFC play a key role in short-term working memory, but the mechani