Structural Biology 领会科学奥秘,把握科学动向 变深奥为通俗,化抽象为形象,Open Online Course for Structural Biology秉承“科学解惑、理性求证”宗旨,致力于为您打造身临其境的线上科普课堂。这里集知识性、思想性、艺术性于一体,让您于动态影音中...
To visualize and analyse molecular structures in their native state and in their cellular context is the overarching aim of in situ structural biology. In the classical structural biology approach purified molecules are investigated isolated from their 'neighbours', far from the intricate macromolecular ...
However, routinely realizing the promise of in situ structural biology requires improved cryo-ET data collection and processing strategies. This becomes particularly apparent when considering the experimental and computational resources needed for cryo-ET compared to the highly efficient cryo-EM pipelines ...
The interspin distances are measured in situ, using pulsed electron–electron double resonance (PELDOR or DEER) spectroscopy. The residual background signals, which are problematic for in situ structural biology, contribute specifically to the intermolecular part of the signal and can be selectively ...
Synchrotron-based infrared imaging is here used to identify early-stage oligomeric/granular aggregated amyloid species in situ in the brain of APP/PS1 transgenic mice and Octodon degus for the first time. Also, APP/PS1 mice show fibrillary aggregates at 6 and 12 months whereas very little ...
Biology of Stem Cells in Human Umbilical Cord Stroma: In Situ and In Vitro SurveysHumanUmbilical cordMesenchymal stem cellsDifferentiationCytoskeletonCells in the umbilical cord stroma have gained attention in recent years; however, differentiation to certain lineages in humans has been demonstrated in ...
Lewis SM, Asselin-Labat ML, Nguyen Q, Berthelet J, Tan X, Wimmer VC, et al. Spatial omics and multiplexed imaging to explore cancer biology. Nat Methods. 2021;18:997–1012. ArticleCASPubMedGoogle Scholar Lubeck E, Coskun AF, Zhiyentayev T, Ahmad M, Cai L. Single-cell in situ RNA...
The appointee will participate in a multidisciplinary project relating to structural characterization of viral-host interactions. He/She will gain experience in structural biology, protein design, virology, and cutting-edge in situ cryo-electron tomography....
Cryo-electron tomography: the challenge of doing structural biology in situ. J. Cell Biol. 202, 407–419 (2013). Article CAS PubMed PubMed Central Google Scholar Trinkaus, V. A. et al. In situ architecture of neuronal alpha-synuclein inclusions. Nat. Commun. 12, 2110 (2021). Article ...
Here, using cryo-FIB and cryo-ET, we delineate the 3D architecture of native chromatin fibres in intact interphase human T-lymphoblasts and determine the in situ structures of nucleosomes in different conformations. These chromatin fibres are not structured as uniform 30 nm one-start or two-...