In situ, literally stands for “in place of”, “at the original site of”, “in the natural position of” or “in the undisturbed shape or orientation of”.
原位培养是相对于室内培养的意思来的,也不完全是说自然条件下,比如养藻,出海在船上用站位的海水来培养,温度光线等一些参数也模拟的差不多,不是完全自然条件下 ex situ 非原位 比如养藻的话就分batch culture还有semi-continuous culture,chemostat等等 Ex situ conservation literally means, "off-site conservation...
InsituPro 概述 InsituPro™是一个用于自动原位杂交(ISH)的专用系统,用于制备检测DNA或RNA(mRNA, miRNA)的样本,也用于检测蛋白质和其他抗原的免疫组织化学(IHC)。该系统自动化了所有繁琐和重复的清洗和孵化步骤的ISH和IHC过程。 特性 工作与整个安装,振动组,载玻片上的薄片,细胞上的盖玻片 广泛应用于生物体(小鼠...
Marine BiologyGarcia-March JR, Jimenez S, Sanchis Solsona MA, Monleon S, Lees J, Surge D, Tena-Medialdea J (2016) In situ biomoni- toring shows seasonal patterns and environmentally mediated gaping activity in the bivalve, Pinna nobilis. Mar Biol 163:1- 2. doi:10.1007/s00227-016-...
Open Online Course | Structural Biology Cryo-Electron Tomography and “molecular sociology ” (Part one) 北京生物结构前沿中心 (Part two) ,赞 点击观看视频 背景介绍 研究“分子社会学”即超大蛋白质机器在生物膜、病毒、细胞甚至组织环境中...
Recently, studies have evaluated early-stage breast lesions using single-cell approaches assaying both genotype21,22and phenotype23, allowing insights into in situ lesion biology that may have been partly obscured by bulk analyses. However, a single-cell transcriptional view of intraepithelial breast le...
Sensitive and spatial exploration of the metabolism of tumors at the metabolome level is highly challenging. In this study, we developed an in situ metabolomics method based on ambient mass spectrometry imaging using air flow-assisted desorption electros
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In Situ Cell Death Detection Kit, POD (细胞原位死亡检测试剂盒, 酶结合法) 是一种用于检测细胞凋亡的实验工具。本文将介绍其实验过程,并引用相关文献。 细胞凋亡是细胞程序性死亡的一种形式,广泛存在于生物体内,对维持组织稳态和生长发育具有重要作用。然而,细胞凋亡的异常也与多种疾病的发生和发展有关,因此,准...
analysis showed that the two haemoglobin subunits were not equivalently expressed, with HBB expressed more than HBA at the protein level (HBA:HBB ≈ 3:5) (Extended Data Fig.1i–k). Next, the expression of haemoglobin in chondrocytes was examined in situ by immunohistochemistry and immunoelectron...