Nucleic acids are in the form of DNA and RNA and are important for cell structure and function. What is a phosphodiester bond in biology? A phosphodiester bond is a covalent bond between phosphate and 2 sugars (hydroxyl groups). A covalent bond involves the sharing of a pair of electrons....
Basic structure of DNA or deoxyribonucleic acid: A nucleotide is the basic of polynucleotide chain of DNA and each nucleotide is composed of three components - i) a nitrogenous base, ii) a pentose sugar and iii) a phosphate group. Nitrogen base is attached to the pentose sugar by an N-gl...
The 9-1-1 DNA checkpoint clamp is loaded onto 5′-recessed DNA to activate the DNA damage checkpoint that arrests the cell cycle. The 9-1-1 clamp is a heterotrimeric ring that is loaded in Saccharomyces cerevisiae by Rad24-RFC (hRAD17-RFC), an alternate clamp loader in which Rad24 rep...
Disease-causing mutations in genes encoding transcription factors (TFs) can affect TF interactions with their cognate DNA-binding motifs. Whether and how TF mutations impact upon the binding to TF composite elements (CE) and the interaction with other TFs is unclear. Here, we report a distinct ...
Answer to: The nucleotides in the backbone of DNA are held together by ___ bond. A) Phosphodiester. B) Peptide. C) Hydrogen. D) Glycosidic...
Proteomic methods for RNA interactome capture (RIC) rely principally on crosslinking native or labeled cellular RNA to enrich and investigate RNA-binding protein (RBP) composition and function in cells. The ability to measure RBP activity at individual b
Cells were then permeabilized in a reagent consisting of 0.5% Triton X-100, 230 μg per mL RNaseA and propidium iodide to 50 μg per mL in phosphate-buffered saline. The samples were kept at 37°C for 30 min followed by flow cytometry analysis (Becton Dickinson FACScan Becton Dickinson,...
For many years, the primary method for phosphopeptide enrichment prior to MS analysis was immobilized metal ion affinity chromatography (IMAC) using the affinity of nega- tively charged phosphate groups on proteins and peptides towards positively charged metal ions (Fe3+, Al3+, Ga3+,...
Fertilization controls various aspects of cereal growth such as tiller number, leaf size, and panicle size. However, despite such benefits, global chemical fertilizer use must be reduced to achieve sustainable agriculture. Here, based on field transcript
A variation of base pairing in nucleic acids, formed by the interaction of the N7 position (as a hydrogen bond acceptor) and C6 amino group (as a donor) of a purine base, with the Watson–Crick (N3–C4) surface of a pyrimidine base. G-quadruplex structures Formed in DNA and RNA mole...