Administration in a hypo- tonic solution is preferable to dose escalation in intraperi- toneal cisplatin chemotherapy for peritoneal carcinomato- sis in rats. Oncology 1999;57:77-82.Tsujitani S,Oka A,Kondo A,et al.Administration in a hypotonic solution is preferable to dose escalation in ...
For spermatocyte chromosome spreads, testes were dissected, and seminiferous tubules were separated and placed in a hypotonic extraction buffer (30 mM Tris-HCl, pH 8.2, 50 mM sucrose, 17 mM sodium citrate, 5 mM EDTA, 0.5 mM DTT, and protease inhibitor cocktail (Roche)) for ...
The total BM cells from WT mice or hdac6 KO littermate were purified and co-stained with FITC-conjugated anti-CD41 antibody and propidium iodide in a hypotonic citrate solution containing 50 μg/mL RNase. The mean ploidy of MKs was measured4. Real-time quantitative RT-PCR The total RNA...
After a brief incubation in hypotonic solution, the preparations were fixed with methanol-glacial acetic acid (3:1). Metaphase cells were banded with Wright stain or with Giemsa trypsin. A minimum of 20 metaphases were analyzed for each specimen. Karyotypes were in accordance with the ...
A hypo-osmolar solution of plasma electrolytes selected from sodium chloride, sodium bicarbonate and electrolytes for injection, the solution having a hypo-osmolarity between 30 and 50% of the osmolarity of plasma. An Independent claim is included for a process for preparing the above solution compr...
(Life Technologies) 1:4000 in a hypotonic lysis buffer (10 mM Tris HCl (pH 8), 5 mM EDTA, 0.1 % Triton X-100) overnight in the dark at 4 °C and then quantified by fluorimetry, measured using a FLUOstar Optima (BMG LabTech, Ortenberg, Germany) set to 485 nm excitation and 535...
The melanin-dispersing principle is more effective in hypotonic solutions than in hypertonic. Furthermore, this hormone is more effective in a solution of a rapidly penetrating molecule such as urea than in a slowly penetrating substance such as sucrose. A monovalent cation is essential for the ...
We investigated changes in whole-cell currents, cell volume, and intracellular calcium concentration ([Ca2+]i) during hypotonic stimulation in whole-cell clamped cultured amphibian renal cells (A6 cells). Upon being exposed to hypotonic solution (80% osmolality), the A6 cells swelled and peaked in...
Supernatant was discarded and 10 ml hypotonic solution (0.075 M KCl) was added, mixed and incubated the cell suspension for 40 min at room temperature. Five drops of chilled freshly prepared Carnoy’s solution (methanol:acetic acid 3:1 at 4 °C) was then added to the cell suspension and ...
Thereafter, cells were detached using trypsin–EDTA, subsequently incubated in 1% hypotonic solution (sodium citrate tribasic dihydrate) and then fixed with Carnoy’s fixative (3:1 methanol to acetic acid) onto glass slides. Q-banded metaphases were analyzed and interpreted according to the ...