Isotonic solution is the solution that has the same water concentration as inside the cell, so there will be no osmotic pressure. Effect of tonicity of solutions on the living cell: Condition 1: If a cell is placed in a hypotonic solution There will be inward movement of water molecules acr...
aincubated in hypotonic KCl solution for 15 min and fixed three times with ice-cold fixative (glacial acetic acid:methanol, 1:3). Two drops of the fixed cell suspension were spread on a clean glass 正在翻译,请等待... [translate]
An animal cell in a ___ solution will shrivel up or crenate. a. hypotonic b. hypertonic If a cell is 30% water and 70% salt and the solution outside the cell is 45% water and 55% salt, where will the water move? Is the solution ...
(i) isotonic solution (ii) hypotonic solution (iii) hypertonic solution (iv) water View Solution Differentiate between the following pairs on the basis of what is mentioned in brackets: Hypotonic solution and Hypertonic solution [condition of a plant cell when placed in them] ...
Briefly, for mitochondria isolation, cells were resuspended in an equal volume ice-cold hypotonic buffer (10 mM NaCl, 1.5 mM MgCl2, 10 mM HEPES, pH 7.5) and allowed to swell for 10 minutes. Cells were homogenized on ice using 20 strokes of the dounce homogenizer B pestle to ...
CTLL/WT and CTLL/703 cells were treated with 10 μg/ml colcemid, centrifuged, and resuspended in hypotonic solution to swell the cells. Cells were then fixed and dropped onto slides. After being stained with Giemsa and dried, slides were analyzed using the Olympus BX-60 microscope. A total...
Cells were treated with a hypotonic solution (0.075 M KCl in ddH2O; Merck) for 30 min at 37 °C and were then fixed with Carnoy’s solution (methanol and acetic acid 3:1; Sigma-Aldrich). For GTG banding, slides were trypsinized (ThermoFisher Scientific) and stained with Giemsa...
1. During exposure to a hypotonic solution, cultured human epithelial cells (Intestine 407) exhibited a regulatory volume decrease (RVD) after initial osmo... A Hazama,Y Okada - 《Journal of Physiology》 被引量: 544发表: 1988年 Endogenous ATP release regulates Cl- secretion in cultured human ...
The cells, irradiated and non-irradiated, were resuspended in 500 μL of a hypotonic solution containing 1X PBS, 0.1% sodium citrate, 0.1% NP-40, 50 μg/mL PI and RNAse A. After 30 min in the dark, samples were acquired on FACSCalibur flow cytometer (BD Bioscences) using Cell Qu...
The total BM cells from WT mice or hdac6 KO littermate were purified and co-stained with FITC-conjugated anti-CD41 antibody and propidium iodide in a hypotonic citrate solution containing 50 μg/mL RNase. The mean ploidy of MKs was measured4. Real-time quantitative RT-PCR The total RNA...