2 × 105/ml cells were incubated in a hypotonic buffer (50 mM Tris (pH 7.4) and 55 mM KCl), fixed with freshly made Carnoy’s solution (75% methanol and 25% acetic acid), dropped onto glass slides and dried at 80 °C. The metaphase plate was revealed by counterstaining with...
Here, red blood cells made semi-spherical by suspension in an hypotonic solution (0.8 Â PBS) and patterned in a (from left to right); (a) 15 mm, (b) 18 mm and (c) 25 mm field (232, 200 and 144 MHz, respectively, at E0.5 W). While geometric considerations determine the ...
Cold storage and deep-supercooled preservation of human red blood cells.aPreservation of human red blood cells (hRBCs) for 42 and 100 days at various temperatures in conventional anticoagulant citrate phosphate double dextrose (CP2D) supplemented with additive solution 3 (AS-3) (CP2D + AS-...
Agents that disrupt the microfilaments, such as the cytochalasins, modify the regulatory responses to both hypotonic and hypertonic stress by inhibiting K+ and Cl− channels that promote RVD and stimulating Na+-K+-2Cl− co-transport responsible for RVI (Chamberlin and Strange, 1989; Mills ...
2C) (63). Notably, septin influence on T-cell shape seems to occur as a response to malformed regions of the plasma membrane, rather than being proactive in preventing such deformations (63). Moreover, cells exposed to hypotonic media, followed by regulatory volume decrease, form a variety ...
solution DMEM Cat. No. SLM-220B) • Complete media (10% FBS in DMEM), and diluted in D-PBS • 1% dimethyl sulfoxide (DMSO) cell freezing media (Hybri-Max™ DMSO Cat. No. D2650) in D-PBS • Cell detachment solution (Accutase® Cat. No. SCR005) • Water, hypotonic,...
hypotonic PI buffer staining and flow cytometric analysis. The percentage of cells in S-phase was determined using the cell-cycle analysis software ModFit. Results indicate means±S.E.M. of seven independent clones in three independent experiments. (d) Cells arrest in G1 after Hoxb8 ...
48 hours after transfection with KLF15 vector, Rat2 cells were lysed on ice in a hypotonic buffer (Hepes 10 mM, MgCl2 1,5 mM, KCl 10 mM, pH 7.9) supplemented with 0.125% NP-40 and protease cocktail inhibitors (Roche, Meylan, France). The lysate was centrifuged for 5 min at 10,000...
FtsZ–CcrZ interaction confirmation by bacterial two-hybrid assay. T25 is the empty vector pST25 and T25–FtsZ corresponds to vector pST25–FtsZ used in combination with pUT18–CcrZ (CcrZ–T18) and pUT18–FtsZ (FtsZ–T18).c, Affinity purification of FtsZ–GFP fromS. pneumoniaecells (se...
Mitoplasts were prepared from the embryonic rat hippocampal neurons, the human endothelium and fibroblast mitochondria by incubation in a hypotonic solution (5 mM HEPES, 100 μμM CaCl22, pH 7.2) for approximately 1 min, and then a hypertonic solution (750 mM KCl, 30 mM HEPES, and 100 μ...