We investigated changes in whole-cell currents, cell volume, and intracellular calcium concentration ([Ca2+]i) during hypotonic stimulation in whole-cell clamped cultured amphibian renal cells (A6 cells). Upon being exposed to hypotonic solution (80% osmolality), the A6 cells swelled and peaked in...
培养皿置于hypotonic KCl解决为15分钟和固定三倍,冰经高温脱腊定纹处理(冰醋酸:甲醇、1:3)。 两滴的固定的细胞暂停是传播关于清洁玻璃 翻译结果5复制译文编辑译文朗读译文返回顶部 正在翻译,请等待... 相关内容 a微笑是很重要的 The smile is very important[translate] ...
Then, we incubated the cells in a hypotonic solution containing 0.4% potassium chloride and 0.4% sodium citrate at 37 °C for 5 min. To analyze the embryonic chromosomes, morulae were transferred to CZB containing 0.25 μg/ mL colcemid and incubated for 3 h and then transferred to a 1:1...
Hypotonicity-induced cell swelling activates TRPA1TRPA1Hypotonic solutionCell swellingCell-attached patch clampHypotonic solutions can cause painful sensations in nasal and ocular mucosa through molecular mechanisms that are not entirely understood. We clarified the ability of human TRPA1 (hTRPA1) to ...
Reconstitution in buffer of choice Disadvantages Time consuming Reproducibility may vary Additives and methods to facilitate physical disruption Cells can be treated with various agents to aid the disruption process. Lysis can be promot...
allowing the material to be carried into the cells via pinocytic vesicles. The cells were transferred to a hypotonic medium for 100 s, which resulted in the release of trapped QDs from the pinocytic vesicles within the cells. The cells were then left in a complete L-15 medium at 28...
hypotonic when places in this type of solution, a red blood cell will swell up and potentially burst pinocytosis "cell drinking" secondary active transport moves substances against their concentration gradients without the direct use of ATP ATP stand for adenosine triphosphate; the energy storing molec...
in hypotonic lysis buffer (10 mM Tris-HCl pH 7.5, 10 mM KCl, 2 mM MgCl2, 33 μL protease inhibitor cocktail (Sigma, #S8830) and 10 μL RNAsin per 10 mL of solution) and homogenized with a tight-fitting pestle for 20 passes. The homogenate is transferred into an ...
For chromatin preparation, cells were lysed in hypotonic lysis buffer (5 mM PIPES pH 8, 85 mM KCl, 0.5% NP40, plus protease inhibitors). Nuclei were recovered by centrifugation, lysed in nuclear lysis buffer (50 mM Tris-HCl pH 8, 1% SDS, 10 mM EDTA, plus protease inhibit...
(Sigma) and washed twice with cold 1X PBS before harvesting. Cytosolic lysates were prepared by incubation of cells 10 min in hypotonic buffer (10 mM Tris-HCl pH 7.4, 0.5 mM MgCl2, 10 mM KCl, 1X CompleteTMEDTA free protease inhibitor (Roche) and 10 u/mL RNAseOutTM(...