Immunoprecipitation detected by flow cytometry (IP-FCM) is an efficient method for detecting and quantifying protein-protein interactions. The basic principle extends that of sandwich ELISA, wherein the captured primary analyte can be detected together with other molecules physically associated within multi...
Interaction of GRP78 with rhLK8 as determined by co-immunoprecipitation and flow cytometry.Interaction of GRP78 with rhLK8 as determined by co-immunoprecipitation and flow cytometry.JinHyung, AhnHyunKyung, YuHoJeong, LeeSoon, Won HongSun Jin, KimJang...
(xi), resulting in immune-complex internalization and removal from the plasma membrane surface. Remaining surface bound gnACHR are enumerated fluorescently by flow cytometry (xii), and compared to maximally and unstained control samples, with the amount of autoantibody present inversely proportional to ...
Ibrahim S, Steinbach F: Immunoprecipitation of equine CD molecules using anti-human MABs previously analyzed by flow cytometry and immunohistochemistry. Vet Immunol Immunopathol 2012, 145 : 7-13. PubMed Abstract Publisher Full TextIbrahim S, Steinbach F: Immunoprecipitation of equine CD ...
Methods Cyclins expression in clinic solid tumors were detected by flow cytometry and post-sorting western blot and confocal microscopy,comparing to the traditional immunohistochemial staining. 方法运用cyclin/DNA双参数流式细胞术及流式细胞术分选后的免疫沉淀蛋白印迹技术和共聚焦显微镜技术检测临床实体瘤细胞...
F13839 FLOW CYTOMETRY SIZE REF KIT 1 KIT F14200 FLUO-4, PENTAPOTASSIUM SA 500 UG F14201 FLUO-4, AM CELL PERMEANT 10 X 50 UG F14204 FLUO-5N, AM CELL PERMEAN 10 X 50 UG F14217 FLUO-4, AM 1 MM SOLUTION 500 UL F14218 FLUO-3, AM 1 MM SOLUTION 1 ML F143 FLUORESCEIN-5-ISOTHI...
(or G) polystyrene beads in solution. The captured peptide is then linked to fluorescently labelled streptavidin and quantified using flow cytometry. The advantage of their assay is that it is readily applicable if a biotinylated antigen peptide is available, but an inherent drawback is that the ...
产口目录编号17-10086 产品名称EZ-Magna ChIP™ A/G Chromatin Immunoprecipitation Kit - (EZ-Magna ChIP™ A/G Chromatin Immunoprecipitation Kit) 选择语言 如果你无法查看MSDS,请使用以下链接下载下载MSDS
4. To calculate the protein concentration in each sample read the absorbance off a BSA standard curve, constructed as follows: prepare serial dilutions of BSA between 2 mg/ ml and 15 mg/ml and add to 100 ml of Bradford reagent in a 96 well plate. ...
J.S.Bridgeman, M.Blaylock, R.E.Hawkins, D.E.Gilham; Development of a flow cytometric co- immunoprecipitation technique for the study of mul- tiple protein-protein interactions and its application to T-cell receptor analysis. Cytometry A, 77, 338- 346 (2010)....