and the cells were mixed and prepared for flow cytometry detection; b) starting up and warming up a flow cytometer system, adjusting the flow speed, then adding PBS-EDTA solution into a sample tube, and flushing a nozzle system of liquid stream; c) the sample of homogenous cells obtained ...
We also found that even if immune cells were constant throughout the menstrual cycle, among the T cell subsets there were differences. CD8+ cells [14.5±9% (mean±S.D.); range 3–30%] were more numerous compared to the mean number of CD4+ cells [7.3±5% (mean±S.D.); range 2–...
(1) The innate and adaptive arms of the immune response are involved in the initiation, development and persistence of atherosclerosis.(2, 3) There is a significant body of evidence that different subsets of the immune cells, such as macrophages, dendritic cells, T and B lymphocytes, are ...
We undertook an investigation in which flow cytometry was used to characterize immune cell populations in the decidua of first-trimester normal pregnancies, spontaneous abortions, and ectopic pregnancies in comparison to the nonpregnant endometrium to demonstrate how the proportions of immunocompetent cell...
Single-Cell Full-Length TCR/BCR Profiling Assay with High Sensitivity and CITE-Seq Compatibility Enables Deeper Immune Cell Characterization Fluorochrome Performance Guide Poster Sorting and Downstream Functional Assessment of Regulatory T Cells Transfer of a 33-Color Flow Cytometry Immunophenotyping Pane...
Fluorescence minus one (FMO) control To adjust for spectral overlap in multicolor experiments Positive control To ensure that the staining procedure is successful FC blocking control To block Fc receptors in immune cells – essential in staining B cells, dendritic cells, monocytes and macrophagesProtein...
RNA flow cytometryUnderstanding how immune cells respond to external stimuli such as pathogens or drugs is a key component of biomedical research. Critical to the immune response are the expression of cell-surface receptors and the secretion of cytokines, which are tightly regulated by gene ...
insights into cellular dynamics involved in disease progression in patients, and in their responses to TKI treatment. This was illustrated in a recent study demonstrating that flow cytometry after immune-staining of membrane markers for aberrant cells served to predict early blast transformation in CML...
Take Advantage of Cytek Biosciences full spectrum flow cytometry systems, offering many advantages over conventional flow cytometers.
Stimulation of cytokine production in immune cells T-cell activation via Concanavalin A T-cell activation with Dynabeads Human T-Activator CD3/CD28 Cell viability protocols for flow cytometry LIVE/DEAD Fixable Dead Cell Stains Protocol LIVE/DEAD Sperm Viability Kit Flow...