The invention relates to the field of flow cytometry and more particularly to a panel of antibody reagents conjugated to fluorescent compounds. Provided are reagent compositions, comprising at least eight distinct fluorochrome-conjugated antibodies comprising a set of at least three identification ...
Single-cell deep phenotyping of IgG-secreting cells for high-resolution immune monitoring. Nat. Biotechnol. 35, 977–982 (2017). Article CAS PubMed Google Scholar Jorgolli, M. et al. Nanoscale integration of single cell biologics discovery processes using optofluidic manipulation and monitoring. ...
The functional analysis of the regulated genes indicated GO terms such as cellular biosynthetic process, translation and mitosis, biosynthetic and metabolic processes, immune response as overrepresented in microglia isolated from glioma-bearing hemispheres compared to controls. In contrast, we found cell-ce...
Microgravity is associated with immunological dysfunction, though the mechanisms are poorly understood. Here, using single-cell analysis of human peripheral blood mononuclear cells (PBMCs) exposed to short term (25 hours) simulated microgravity,
In this study, we performed a comprehensive battery of immune phenotyping, whole-genome microarray analysis, and microRNA expression profiling of GAMs with matched blood monocytes, healthy donor monocytes, normal brain microglia, nonpolarized M0 macrophages, and polarized M1, M2a, M2c macrophages. ...
Taking advantage of the Berlin BASE-II study, here we have undertaken detailed phenotyping of peripheral γδ T-cells in 73 younger and 144 older individuals, in relation to their CMV-serostatus. At the same time, we assessed similar parameters for the αβ T-cell subset in comparison in ...
Phenotyping studies Flow cytometric determinations of BM CD34+ cell counts were undertaken in Halifax as recommended by the International Society for Cellular Therapy [27] by using CD45-fluorescein isothiocyanate (FITC) and CD34-phycoerythrin (PE) monoclonal antibodies from StemKit (Beckman Coulter, Mia...
When working with large cohort sizes, immune-phenotyping by flow cytometry is time consuming and highly work intensive. We have developed improved workflows combining automated tissue dissociation with novel TIL specific isolation reagents to allow for more accurate and faster analysis of TILs and TIL ...
Isolation and phenotyping of immune cells by flow cytometry were done as previously described42. Briefly, single cell suspensions were prepared from spleens by mechanical disruption via forcing through 40 µm cell strainers (Thermo Fisher Scientific). For the isolation of lymphocytes from the sma...
The development of innovative immune cell therapies relies on efficient cell tracking strategies. For this, multiscale fluorescence-based analyses of transferred cells into the host with complementary techniques, including flow cytometry for high-throughput cell analysis and two-photon microscopy for deep ...