Figure 1.Principle of flow cytometry. Cells (red) flow one by one past the beam from a laser (gray), and the resulting light signals (various colors) are spectrally separated by filters (cyan) and detected by photodiodes and photomultipliers (pink). Image created using BeamWise modeling softw...
Modern developments in flow cytometry, combined with the ongoing emergence of novel fluorescent labels have enabled an unprecedented level of analysis in cell biology, immunology, and microbiology, as well as, cancer and clinical research studies. With these tools, distinct populations of cells can be...
Flow cytometry (FCM) is an analytic technique that is capable of detecting and recording the emission of fluorescence and light scattering of cells or particles (that are collectively called “events”) in a population1. A typical FCM experiment can produce a large array of data making the anal...
and presence of surface membrane or intracellular antigens or molecules. the cells are suspended in fluid in which movement is controlled by the size and configuration of tubing, chambers, and pumps specific to the flow cytometry instrument. the pattern of light signals produced from the laser lig...
Most advances in flow cytometry have depended on co-creation, with scientists, research institutions and companies working together. In 1953, American electrical engineer Wallace Coulter received a patent for the Coulter Principle, a technique for analyzing particles in a fluid. In the 1970s, the ...
Flow cytometry is a high throughput method extensively used in both experimental and clinical settings for characterizing cell phenotypes, especially in the disciplines of hematology, immunology and infectious diseases. In flow cytometry, intracellular or cell surface molecules (markers) in a cell populati...
Flow cytometry, an overview Flow cytometry had its origins in the late 1960s with the development of the first cell sorter based on the principle of droplet deflection used by Varian ... LS Cram - 《Methods in Cell Science An Official Journal of the Society for in Vitro Biology》 被引量...
The flow cytometry-based assay for TCA was sensitive and accurate and had potential value for clinical application. 1. Introduction The complement system consists of more than 30 compo- nents, which are widely present in normal human or verte- brate serum, tissue fluid, and cell membrane ...
whereas alloreactive T cells are generally estimated to represent 5-15% of the total T-cell repertoire29,30,31,32. Importantly, we showed that imaging flow cytometry can produce a very comparable measure of alloreactive T-cell frequency23and that changes in synapse frequency within a polyclonal ...
Compensating in flow cytometry is an unavoidable challenge in the data analysis of fluorescence-based flow cytometry. Even the advent of spectral cytometry cannot circumvent the spillover problem, with spectral unmixing an intrinsic part of such systems.