*Po0.05, **Po0.001 and ***Po0.0001 (Student's t-test). Data are representative of two experiments with four mice per group (error bars, s.e.m.). amount of BAFF-R, TACI and BCMA, compared with PD-L1lo and PD-L1int B cells. Crucially, PD-L1hi B cells from aCD20 mAb-treated...
(Fig.4F–I), with long-term administration of LD4172 showing no impact on mouse body weight (Fig.4J). To test whether inhibition of RIPK1 kinase activity also enhances tumor responses to ICB therapy, we treated B16F10 xenograft tumors with the RIPK1 kinase inhibitor T2I, alone or in ...
All results were presented as mean ± SD. The one-way analysis of variance (ANOVA) with Tukey’s post-hottest was used for multiple comparisons (when more than two groups were compared). The logrank test was used in survival analysis. All statistical analyses were carried out with the ...
The cells were inoculated on 24-well plates and treated with 10 nM paclitaxel. The culture supernatant was collected and centrifuged at 1000 rpm for 10 min. Collect the supernatant and transfer it to a 96-well plate for detection according to CytoTox 96 test kit requirements. The formula for...
10). Fig. 4: Validation of IASNDS-induced GBM pyroptosis and ICD-related changes in vitro. a Visualization of cellular pyroptosis changes induced by the IASNDS under confocal microscopy, showing membrane pores and vacuole formation in QL01#GBM cells (n = 3 independent experiments). b ...
An autologous serum skin test (ASST) was performed on 138 patients and was positive in 58 (42.02%). Results: A questionnaire and informed consent, approved by the Research Ethics Office, was sent to patients and replies were received from 101 participants. We were unable to contact 25 ...
Under 808 nm NIR irradiation, the PB-Mn/OVA NEs with outstanding photothermal conversion efficiency of 30% significantly destroyed tumor cells by inducing immunogenic cell death (ICD). Impressively, the PB-Mn/OVA NEs could activate the cGAS-STING pathway to promote the maturation and the ...
Statistical analysis by one-way ANOVA with Turkey’s multiple comparisons test. Source data are provided as a Source data file. Full size image To clarify the mtDSN-2-induced ICD mechanism, we examined reactive oxygen species (ROS) production and ER stress, as they are essential prerequisites ...
images of calcein-AM fluorescence and the quantitative detection of cell viability in different cells. Scale bar, 200 μm. The data are presented as the mean ± SD;n = 6. At testwas performed for statistical analysis (***:P < 0.0001). ICD: immunogenic cell death; CRT: ca...
Statistical analysis was performed using two-way ANOVA followed by Sidak’s multiple comparisons test, with significance levels indicated. Full size image Intraperitoneal (i.p.) administration of LD4172 (10 mg/kg) to C57BL/6J mice led to Cmax, t1/2, and AUC as 2.9 µM, 1.5 h...