Analysis of the differences between the groups was performed by non-parametric statistics (Fisher’s exact test and Wilcoxon’s rank sum test). Results The groups did not differ for age, schooling and disease duration (from diagnosis), however, PD patients were mostly males, and with fewer com...
It was shown that PTM-induced immunogenic cell death (ICD) stimulates the adaptive immune system [10, 15], but further research is crucial to understand the reactive species in NSCLC fully and to develop more effective immunotherapies. In the present study, we chose the Lewis lung cancer (LLC...
images of calcein-AM fluorescence and the quantitative detection of cell viability in different cells. Scale bar, 200 μm. The data are presented as the mean ± SD;n = 6. At testwas performed for statistical analysis (***:P < 0.0001). ICD: immunogenic cell death; CRT: ca...
∗p < 0.05 (Holm-Sidak’s multiple comparisons test). Discussion ICD is a functionally peculiar form of regulated cell death that activates an adaptive immune response specific for endogenous or exogenous antigens expressed by dying cells.29,30,31 In cancer therapy, studies have reported the ...
*Po0.05, **Po0.001 and ***Po0.0001 (Student's t-test). Data are representative of two experiments with four mice per group (error bars, s.e.m.). amount of BAFF-R, TACI and BCMA, compared with PD-L1lo and PD-L1int B cells. Crucially, PD-L1hi B cells from aCD20 mAb-treated...
10). Fig. 4: Validation of IASNDS-induced GBM pyroptosis and ICD-related changes in vitro. a Visualization of cellular pyroptosis changes induced by the IASNDS under confocal microscopy, showing membrane pores and vacuole formation in QL01#GBM cells (n = 3 independent experiments). b ...
The bacterial index, the tuberculin skin test and research for HIV were negative. The diagnosis of borderline tuberculoid leprosy was established. The number of TCD4+ cells was lower before (572 cells/mm3) than after (665 cells/mm3) skin lesions detection. We found no previous reports in ...
Under 808 nm NIR irradiation, the PB-Mn/OVA NEs with outstanding photothermal conversion efficiency of 30% significantly destroyed tumor cells by inducing immunogenic cell death (ICD). Impressively, the PB-Mn/OVA NEs could activate the cGAS-STING pathway to promote the maturation and the ...
(Fig.4F–I), with long-term administration of LD4172 showing no impact on mouse body weight (Fig.4J). To test whether inhibition of RIPK1 kinase activity also enhances tumor responses to ICB therapy, we treated B16F10 xenograft tumors with the RIPK1 kinase inhibitor T2I, alone or in ...
The cells were inoculated on 24-well plates and treated with 10 nM paclitaxel. The culture supernatant was collected and centrifuged at 1000 rpm for 10 min. Collect the supernatant and transfer it to a 96-well plate for detection according to CytoTox 96 test kit requirements. The formula for...