invasively measured neuralphenotypes, offering a rich framework for evaluating pathophysiological hypotheses and putative mechanisms.Here, we overview some fundamental methods in imaging transcriptomics and outline their application tounderstanding brain disorders of neurodevelopment, adulthood, and neurodegeneration....
Highly sensitive spatial transcriptomics using FISHnCHIPs of multiple co-expressed genes Article Open access 15 March 2024 Spatial multi-omics at subcellular resolution via high-throughput in situ pairwise sequencing Article 14 May 2024 Spatial tissue profiling by imaging-free molecular tomography ...
Imaging transcriptomics of the brain for schizophrenia û收藏 转发 2 ñ赞 评论 o p 同时转发到我的微博 按热度 按时间 正在加载,请稍候... 查看更多 a 28关注 27粉丝 165微博 微关系 他的关注(28) tongluobing 啊噜哇689 啊庆婚姻中介13829431170 小11you 他...
introduce Composite In Situ Imaging (CISI), a method that leverages structure in gene expression across both cells and tissues to limit the number of imaging cycles needed to obtain spatially resolved gene expression maps. CISI defines gene modules that can be detected using com-posite measurements ...
Recent advances in imaging-based spatially resolved transcriptomics (im-SRT) technologies now enable high-throughput profiling of targeted genes and their locations in fixed tissues. Normalization of gene expression data is often needed to account for te
Image-based approaches to single-cell transcriptomics, in which RNA species are identified and counted in situ via imaging, have emerged as a powerful comp... JR Moffitt,J Hao,G Wang,... - 《Proceedings of the National Academy of Sciences of the United States of America》 被引量: 66发表...
(e.g., RNAscopeTM, LGC StellarisTM, etc.) are limited to 3 or 4 targets. Other emerging spatial transcriptomics technologies such as seqFISH can offer greater multiplexing capabilities but requires many rounds of sample re-labeling, imaging, indexing, and error-prone image registration. The ...
I review recent technological advancements in coupling single-cell transcriptomics with cellular phenotypes including morphology, calcium signaling, and el
Genet. 12:636743.doi: 10.3389/fgene.2021.636743DEEPsc: A Deep Learning-BasedMap Connecting Single-CellTranscriptomics and Spatial ImagingDataFloyd Maseda 1 , Zixuan Cang 1,2 * and Qing Nie 1,2,3 *1 Department of Mathematics, University of California, Irvine, Irvine, CA, United States, 2 ...
Imaging transcriptomics investigates the relationship between neuroanatomical/neuroimaging features and gene expression. The spatial and temporal distribution of the expressed genes and their pattern of spreading over time can contribute to elucidating cellular and molecular processes involved in neurodegeneration....