A one-stop shop for 3D spatial transcriptomics Tiago Faial Research Highlights15 Jul 2024 Nature Genetics Volume: 56, P: 1327 CRISPRdelight illuminates non-repetitive genomic loci in living cells We demonstrate CRISPRdelight, a robust CRISPR–Cas12a-based method for imaging non-repetitive genom...
Subcellular spatial transcriptomics cell segmentation (SCS) combines information from stained images and sequencing data to improve cell segmentation in high-resolution spatial transcriptomics data. Hao Chen ,Dongshunyi Li &Ziv Bar-Joseph This Month|11 May 2023 ...
the pathological mechanisms underlying AD are still far from well understood. Apart from the development of methods, the integration of multimodal imaging data and genomics, microRNAomics, metabolomics, proteomics, and transcriptomics will continue to be an...
van Aarle, W. et al. Fast and flexible X-ray tomography using the ASTRA toolbox.Opt. Express24, 25129 (2016). ArticlePubMedGoogle Scholar van Aarle, W. et al. The ASTRA Toolbox: A platform for advanced algorithm development in electron tomography.Ultramicroscopy157, 35–47 (2015). Articl...
RNA samples are investigated using RT-PCR or transcriptomics, while serum samples and cell supernatants are analysed with ELISA, Luminex or Figure 6 FLAIR and T2* images. (A) periventricular hyperintensities (PWH) and (B) deep white matter (DWM) lesions on FLAIR. (C) T2* image showing ...
Capturing the transient cellular or subcellular dynamics and interactions across multiple pathological or physiological states with 2pSAM, together with advanced spatial transcriptomics technology,16,68 offer great opportunities to better understand how living systems organize and respond to different stimuli ...
A single–cell type transcriptomics map of human tissues 2021, Science Advances Neuroinvasion of SARS-CoV-2 in human and mouse brain 2021, Journal of Experimental Medicine Assembly of functionally integrated human forebrain spheroids 2017, Nature Advanced CUBIC protocols for whole-brain and whole-body...
RNA samples are investigated using RT-PCR or transcriptomics, while serum samples and cell supernatants are analysed with ELISA, Luminex or Figure 6 FLAIR and T2* images. (A) periventricular hyperintensities (PWH) and (B) deep white matter (DWM) lesions on FLAIR. (C) T2* image showing ...
Extending EM analysis with techniques conceptually equivalent to the fluorescence toolbox will allow full analysis of the now automatically acquired large volumes of data and would therefore radically improve its utility in biology. X-rays are generated during EM imaging and, when collected in high ...
(Fig.3f), and spatial transcriptomics data from a study by Erickson et al.54(Fig.3g). While this did not have an apparent effect on the intracellular lactate pool, a reduction in the total LDH expression in tumour epithelium may be functionally consistent with its increased capacity for ...