https://doi.org/10.1016/j.neuron.2012.02.011Get rights and content Under an Elsevier user license open archiveCalcium ions generate versatile intracellular signals that control key functions in all types of neurons. Imaging calcium in neurons is particularly important because calcium signals exert their...
Calcium imagingimagingNeuronneuron deathApoptosisapoptosisHippocampalhippocampal neuronsneuronsFura2/AMFura2/AMIntracellularIntracellular ++ is involved in control of a large variety of cell functions including apoptosisapoptosis and neuronneuron cell deathneuron . For example, + overloadoverload is critical ...
Calcium imaging with genetically encoded calcium indicators (GECIs) is routinely used to measure neural activity in intact nervous systems. GECIs are frequently used in one of two different modes: to track activity in large populations of neuronal cell b
Imaging Calcium in Neurons. Neuron 73, 862–885 (2012). Article CAS PubMed Google Scholar Peterka, D. S., Takahashi, H. & Yuste, R. Imaging voltage in neurons. Neuron 69, 9–21 (2011). Article CAS PubMed PubMed Central Google Scholar Sheth, S. et al. Evaluation of coupling ...
2016. Calcium imaging of neural circuits with extended depth-of-field light-sheet microscopy. Optics Letters 41. doi: 10.1364/OL.41.000855Quirin, S. et al. Calcium imaging of neural circuits with extended depth-of-field light- sheet... S Quirin,N Vladimirov,CT Yang,... - 《Optics Letters...
Deretter bytte til stor forstørrelse og fluorescens bildebehandling for å finne og fokusere på spesifikke neuron. Stimulus: Hvis en nerveaktivitet ved en spesifikk stimulus ønskes (lys, elektrisk felt, gustatory og olfactory signaler, temperatur etc.), må stimulus anordningen v...
Complex dynamic cellular networks have been studied in physiological and pathological processes under the light of single-cell calcium imaging (SCCI), a method that correlates functional data based on calcium shifts operated by different intracellular and extracellular mechanisms integrated with their cell ...
Human cellular models of medium spiny neuron development and Huntington disease Monika M.Golas, inLife Sciences, 2018 5.5Calcium imaging The calcium state of cells can be examined bycalcium imagingwith fluorescent calcium indicator molecules [216,217]. Once calcium binds to a chemical [216] or pol...
High Ca2+-phosphate transfection efficiency in low-density neuronal cultures. Nat. Protoc. 1, 695–700 (2006). Article CAS PubMed Google Scholar Wardill, T. J. et al. A neuron-based screening platform for optimizing genetically-encoded calcium indicators. PLoS ONE 8, e77728 (2013). ...
Our work illustrates the interest of spectral analysis for the study ofin vitronetworks, and its potential use as a network-state indicator, for instance to compare healthy and diseased neuronal networks. 展开 关键词: neuronal cultures multineuron calcium imaging spectral analysis network development ...