under a microscope The total number of cells in the field of view was 265.The number of cells at the different stages of the cell cycle are shown in Table 23.Stage of cellcycle Number of cells interphase 207prophase 42metaphase 4anaphase 6telophase 6Table 23The cell cycle takes 20 hours....
The aim of the present investigation was to visualize differences resulting in the texture of the chromatin fibre from metaphase chromosomes after each digestion using digital image analysis (DIA) facilities. To this purpose, metaphase chromosomes derived from a L-929 mouse cell line were digested ...
This step describes the generation of an array of ∼4,000 yeast strains, by introducing the three fluorescent cell cycle positional markers of the Cell Cycle Query Strain described in the “before you begin” section, into each strain of the Yeast ORF-GFP Collection. This is achieved using ...
The primary cell walls in a 500 nm–thick apple leaf section identified with an antibody to the methyl-esterified regions of pectic polysaccharides or pectin, and visualized with green-fluorescent Alexa Fluor® 488 goat anti–rabbit IgG antibody (Cat. No. A11008). The orange regio...
Visualizing temporal dynamics of phospho-Smad complexes using semi-automated isPLA We set out to establish a semi-automated isPLA protocol to investigate cellular signaling events at the single-cell level in high-throughput (Fig. 1a). For this purpose, we grew and fixed HaCAT keratinocyte cells ...
The pattern of volume growth of Saccharomyces cerevisiae a/alpha was determined by image cytometry for daughter cells and consecutive cycles of parent cell... CL Woldringh,PG Huls,NO Vischer - 《Journal of Bacteriology》 被引量: 203发表: 1993年 Three‐dimensional DNA image cytometry by confoca...
The system enables interactive data exploration for image-based screens and automated scoring of complex phenotypes that require combinations of multiple measured features per cell.Similar content being viewed by others RefCell: multi-dimensional analysis of image-based high-throughput screens based on ‘...
The health care sector is totally different from any other industry. It is a high priority sector and consumers expect the highest level of care and services regardless of cost. The health care sector has not achieved society’s expectations, even though
In almost 25 years of cell detection software development, there is still no single piece of commercial software that works well in practice when applied to early mouse embryo or stem cell image data. To address this need, we developed MINS (modular interactive nuclear segmentation) as a MATLAB...
The formation of a metaphase spindle, a bipolar microtubule array with centrally aligned chromosomes, is a prerequisite for the faithful segregation of a cell's genetic material. Using a full-genome RNA interference screen of Drosophila S2 cells, we identified about 200 genes that contribute to spi...