of the dichroic mirrors have been designed in such a way that only the desired excitation wavelengths are deflected downwards through the objective onto the specimen, while the unwanted wavelengths are transmitted by the dichroic mirror and collected in a light trap [21] behind the dichroic mirror...
Illumination device for use in microscope uses motor to rotate mirror for selectively switching light source into illumination beam pathdoi:DE102004064115 B4A rotatable mirror arranged on motorized mirror housing (5), selectively switches one of the three light sources (7) into an illumination beam ...
PROBLEM TO BE SOLVED: To provide a vertical illumination optical system for a microscope having an adjusting function to adjust the image position of a light source to the rear focal position of each microscopic objective lens in response to replacement of the microscopic objective lens with a sim...
A wide-field fluorescence microscope is typically a partially coherent, low-pass filtering imaging system with an optical transfer function (OTF) support that has a torus-like shape21,22. The system’s lateral and axial resolutions can be described as 2sin(α)n/λand [1-cos(α)]n/λ, re...
Using a digital mirror device a... Dongli,Xu,Tao,... - 《Journal of Biomedical Optics》 被引量: 35发表: 2013年 Superresolution Multidimensional Imaging with Structured Illumination Microscopy The resolution of an optical microscope is fundamentally limited by diffraction. In a conventional wide-...
scattering. To validate our instrument, we measured microbeads mounted on a reflective substrate embedded in immersion oil (Fig.1b, c). When imaged on a reflective surface (mirror), the phase shift is twice the value in a transmission configuration35. The expected phase shift from the bead ...
are classified as near-vertical illuminators. In stereomicroscopes of the Greenough design, a mirror is located directly between the two eye paths at the base of the microscope body, and directs light from the source downward, nearly vertically, to the specimen surface. Current Nikon stereomicrosco...
Darkfield observation in stereomicroscopy requires a specialized stand containing a reflection mirror and light-shielding plate to direct an inverted hollow cone of illumination towards the specimen at oblique angles. The principal elements of darkfield illumination are the same for both stereomicroscopes ...
The axial resolution of three-dimensional structured illumination microscopy (3D SIM) is limited to∼300 nm. Here we present two distinct, complementary methods to improve axial resolution in 3D SIM with minimal or no modification to the optical system. We show that placing a mirror directly ...
for different optical properties, it is provided according to the invention that the optics arranged after the micro light source(s) has at least one variable-focus lens element (vario lens). In addition, is an optical observation device is described—for example, an ophthalmologic microscope. ...