Illumina 测序平台介绍 博奥生物基于Genome AnalyzerIIx,采用最新的TruSeq试剂,推出了小RNA测序(small RNA sequencing)、转录组测序(tanscriptome sequencing)、基于测序的基因表达研究(gene expression profiling based on sequencing)、全外显子组测序(exome
Small RNA libraries were prepared from ten mouse organs (brain, lung, liver, spleen, kidney, heart, skin, muscle, testisand white blood cells) using the AIR Small RNA Sequencing Kit and the conventional preparation method. These samples were prepared using both total RNA and small RNAs ...
博奥生物基于Genome AnalyzerIIx,采用最新的TruSeq试剂,推出了小RNA测序(small RNA sequencing)、转录组测序(tanscriptome sequencing)、基于测序的基因表达研究(gene expression profiling based on sequencing)、全外显子组测序(exome sequencing)、目标区域深度测序(targeted sequence deep sequencing)、DNA甲基化测序(DNA...
目前我们主要分析的数据还是二代测序的数据,也就是大家经常挂在嘴边的NGS,而这其中最大的赢家应该算是illumina测序公司了,其经典的边合成边测序(sequencing by synthesis,SBS)巧妙地利用带不同荧光的dNTP来让碱基组成可视化,本身还是很有意思的。但随之而来的就有一些问题,比如以RNA-seq为例,如果你是一个经典的从表...
The Small RNA Library Prep Kit for Illumina consists of all the reagents and components required to generate small RNA libraries to be used for next-generation sequencing on an Illumina platform. All molecular reagents including adaptors, primers, enzyme mixes and buffers are provided. A purificatio...
1、样品类型:完整且无污染的总RNA; 2、样品需求量(单次):≥10 μg; 3、样品浓度:≥200 ng/μL; 4、样品纯度:OD260/280 =1.8~2.2;OD260/230≥2.0;28S:18S≥1.5;RIN≥8.0;23S:16S≥1.5 (此项要求只针对原核生物)。 参考文献 [1]Li D, Wang L, Liu X, et al. Deep Sequencing of Maize Sm...
Transcriptome sequencing (total RNA-Seq, mRNA-Seq, gene expression profiling) checkmark checkmark Targeted gene expression profiling checkmark checkmark checkmark checkmark checkmark miRNA & small RNA analysis checkmark checkmark checkmark checkmark ...
2、序法推出了GenomeSequencer20System(GS20)系统,开启了边合成边测序(sequencing-by-synthesis)高通量测序的进程。2007年,罗氏公司收购了454,并推出了一系列性能更优的NG繇统,极大的提升测序通量和准确性,也进一步增加了测序读长。虽然罗氏454具有读长优势,但是测序通量和成本始终限制了454平台的推广,因此罗氏在2016...
1. 加入测序引物(sequencing primer)、四种 dNTP、DNA 聚合酶。 测序引物结合到靠近 P5 的测序引物结合位点 1(sequencing primer binding site 1)上。在聚合酶的作用下,与 Forward strand 相应位置碱基配对的 dNTP 就会结合到新合成的链上,而由于叠氮基的存在,后面的 dNTP 无法继续连接。这时用水将剩余的 dNTP ...
Method miRNA and Small RNA Sequencing" Illumina/TruSeq Small RNA Library Prep Kit -Set B (24 rxns) (Set B: indexes 13-24)/RS-200-0024/1 Ea RS-200-0024 Illumina 产品编号: RS-200-0024美 元 价: $2450.00会 员 价: 待定品 牌: Illumina产 地: 美国公 司: Illumina, Inc.产品分类: 分...