Illumina sequencing and array technologies drive advances in life science research, translational and consumer genomics, and molecular diagnostics.
Illumina's Next-Generation Sequencing (NGS) workflow is a sophisticated and highly efficient process that enables researchers to unravel the mysteries of genetics with speed and accuracy. The workflow on Illumina platforms can be broken down into three main steps: library preparation, sequencing, and...
For RNA sequencing (RNA-Seq) data, additional steps may also be required during cleanup. Correction of sequence bias introduced during library preparation (e.g., from cDNA synthesis [6]). Note that sequence bias can also be introduced from...
1、1Illumina测序的原理和应用2Illumina测序流程 Data Analysis 数据分析 Sequencing 测序 Cluster Generation 簇生成 Library Preparation 文库制备测序流程3Adapter and Library 接头和文库接头:与flow cell结合区Read1和Read2测序引物结合区Index 用来区分不同样本4Illumina测序流程 Data Analysis 数据分析 Sequencing 测序...
Sequencing Method方法 mRNA SequencingmRNA 测序 Automation Capability Liquid Handling Robots ——Truseq RNA文库构建原理 dT磁珠抓取含polyA尾的mRNA,从总RNA中提取mRNA。 逆转录技术合成cDNA。 PCR法扩增DNA产物。 ——TruSeq RNA Library Preparation Kit v2组份和保存 ...
Conventional Illumina RNA-Seq does not have the resolution to decode the complex eukaryote transcriptome due to the lack of RNA polarity information. Strand-specific RNA sequencing (ssRNA-Seq) can overcome these limitations and as such is better suited for genome annotation, de novo transcriptome ass...
Over 85% of the insertions can be confirmed by the genomic sequencing data (Fig. 5F). 14 out of the 21 insertions located within an existing protein-coding exon can be fully or partially validated with the RNA-seq data (Fig. 5; Supplementary Table S7). To illustrate the impact ...
RNA, miRNA, siRNA RNA Sequencing RNA 测序中常用名词解释 Total RNA rRNA-depleted RNA Ribosomal RNA (rRNA) is 80% of total RNA Removal of rRNA is called rRNA-depleted RNA Due to the size selection step, Small RNAs are not present in the final library Poly(A) ...
Streamlined RNA-Seq solution for clear and comprehensive analysis across the coding and non-coding transcriptome with exceptional study flexibility.
The Small RNA Library Prep Kit for Illumina consists of all the reagents and components required to generate small RNA libraries to be used for next-generation sequencing on an Illumina platform. All molecular reagents including adaptors, primers, enzyme mixes and buffers are provided. A purificatio...