IL-10 mutations are associated with an increased susceptibility to HIV-1 infection and rheumatoid arthritis. This antibody is a neutralizing control antibody for IL-10, the immunogen is the same as Neutrakine 69018-1-Ig but could not neutralize human IL-10....
neutralizing antibodynoncompetitiveprotein engineeringIL-10 is a dimeric cytokine that must engage its high-affinity cell surface receptor, IL-10R1, to induce multiple cellular activities. Here we report the 1.9 crystal structure of an engineered IL-10 monomer (IL-10M1) in complex with a ...
ELISA - Anti-IL10 antibody [B-S10] - Azide free (ab47222)Image courtesy of an anonymous Abreview. We examined the effect of anti IL-10 to study the regulatory role of IL-10 and the interaction of IL-10 with its receptor. Measurement of neutralizing activity of anti IL-10 by TNFa-EL...
To further confirm the role of IL-10 in PMC remodeling, small molecule inhibitor (AS101) and neutralizing antibody of IL-10 (anti-IL-10) was used to inhibit serum IL-10. As shown in Fig. 3H-K, SLE-PT serum induced collagen-I and α-SMA synthesis in primary rat PMCs, but these...
Finally, the antidepressant effect of microglial Pdcd4 knockout under LPS-challenged conditions was abolished by intracerebroventricular injection of the IL-10 neutralizing antibody IL-10Rα. Our study elucidates the distinct involvement of microglial Pdcd4 in neuroinflammation, suggesting its potential as...
which use IL-10 to reduce proinflammatory responses. IL-10 is also produced by a subset of CD4+T regulatory cells. Various manipulations of IL-10 levels with repeated administrations of anti-IL-10 neutralizing antibodies, genetic ablation or injections of recombinant cytokine have shown contradictory...
Third, we examined the effects of an IL-10 neutralizing antibody. The antibody prevented the enhanced expression of Arg1, CD206 and IL-4Rαby TonEBP knockdown (Fig. 4c). In aggregate, these data demonstrate that TonEBP suppresses M2 genes via down regulation of IL-10 in macrophages....
and that production of IL-10 limits antiviral responses by preventing the formation of neutralizing antibodies (and possibly cellular immunity as well) [17]. Relevant to the IL-10−/−model, colitis is abrogated in germ-free conditions and ameliorated by changing the balance of intestinal flora...
Neutralizing anti-IL-10 monoclonal antibody (anti-IL-10 mAb) exactly mimicked the effects of PI3K inhibitors to enhance IL-12 p70 production. The enhancement effect of PI3K inhibitors on IL-12 p70 production almost completely disappeared by the treatment with anti-IL-10 mAb. PI3K inhibitors ...
Further analysis shows that the IL-10–neutralizing antibody inhibited IL-10–induced STAT3 activation, resulting in inhibition of PD-L1 expression in DLBCL cells (Figure 2D). Downregulation of STAT3 via siRNA approaches led to PD-L1 protein inhibition (Figure 2E), as well as blocking STAT3 ...