Total RNA was isolated from highly pure, sorted human ILC subsets using the TRIZOL reagent according to the manufacturer’s instructions (Invitrogen). Final preparation of RNA was considered DNA- and protein-free if the ratio of readings at 260/280 nm was ≥1.7. Isolated mRNA was reverse-tr...
Chromium Next GEM Single Cell 5’ Kit v2 (10× Genomics) (PN-1000263) was used to perform single-cell separation and library construction according to the manufacturer’s instructions. Briefly, the cell suspension, barcoded gel beads and partitioning oil were loaded onto the 10x Genomics ...
42 Briefly, feces were collected from mice and fungal genomic DNA was isolated from the feces and from colons with use of the QIAamp DNA Stool Mini Kit (Qiagen) according to the manufacturer’s instructions. Fungal 18S rDNA was subsequently evaluated by using qPCR analysis. Fungal burden was ...
Using cDNA as a template and β-actin as an internal reference, the cDNA template obtained by reverse transcription was added to the detection system according to the RT-PCR reagent operating instructions. The ABI7500 fluorescence RT-qPCR system (Applied Biosystems, Germany) was used to detect ...
Unconventional protein secretion represents an important process of the inflammatory response. The release of the pro-inflammatory cytokine interleukin (IL)-1β which burst during pyroptosis as a consequence of gasdermin D plasma membrane pore formation,
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