After 36 h coculture, TCR-T or IL-21R-TCR-T were stained with TCR-PE antibody and sorted by EasySepTM Release PE Positive Selection Kit (Stemcell Technologies) following the instructions. Single-cell RNA sequencing ScRNA-seq libraries were prepared and sequenced by Genergy Biotechnology (...
Using cDNA as a template and β-actin as an internal reference, the cDNA template obtained by reverse transcription was added to the detection system according to the RT-PCR reagent operating instructions. The ABI7500 fluorescence RT-qPCR system (Applied Biosystems, Germany) was used to detect ...
Fig. 4 Induction ofDusp10suppresses IL-5 production in ILC2.aSchematic representation of wild-typeDusp10(WT) and C409S mutant.b–gFreshly prepared ILC2s purified form the spleen of IL-33-injected BALB/c mice were infected with a Mock (empty vector),Dusp10, or C409S mutant-IRES-hNGFR...